Abstract
The protein refractive index was observed to increase by 8 · 10–6 during proteolysis of bovine serum albumin (BSA, 4 mg/mL) with pepsin (2.2 mg/mL). It was believed earlier that the refractive index of a protein solution depends only on the protein concentration and amino-acid composition and remains unchanged during protein hydrolysis. An increase in the refractive index during proteolysis in solution was detected using an original laser interferometer with an improved sensitivity. The interferometer makes it possible to measure the difference in refractive index between solutions in two cells to an accuracy of approximately 6 · 10–7.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- BSA:
-
bovine serum albumin
- TCA:
-
trichloroacetic acid
References
H. Zhao, P. H. Brown, M. T. Magone, et al., J. Mol. Biol. 411 (3), 680 (2011).
H. Zhao, P. H. Brown and P. Schuck, Biophys. J. 100 (9), 2309 (2011).
D. C. Yin, Y. Inatomi, H. M. Luo, et al., Meas. Sci. Technol. 19 (045303), 1 (2008).
E. Snell, J. Helliwell, T. Boggon, et al., Acta Crystallogr. D 52 (3), 529 (1996).
T. Cole, A. Kathman, S. Koszelak, et al., Anal. Biochem. 231 (1), 92 (1995).
D. J. Bornhop, J. C. Latham, A. Kussrow, et al., Science 317 1732 (2007).
S. T. Jepsen, T. M. Jørgensen, W. Zong, et al., Analyst 1 (2012).
V. N. Binhi and R. M. Sarimov, Biophysics (Moscow) 59 (4), 515 (2014).
V. N. Ingram, Nature 4237 83 (1951).
http://web.expasy.org/peptide_mass/
R. Barer and S. Joseph, Q. J. Microsc. Sci. 95 (4), 399 (1954).
T. L. McMeekin, M. Wilensky, and M. L. Groves, Biochem. Biophys. Res. Commun. 7 (2), 151 (1962).
T. L. McMeekin, M. L. Groves, and N. J. Hipp, in Amino Acids and Serum Proteins, Ed. by J. Stekol (American Chemical Society, Washington, DC, 1964).
V. Ball and J. J. Ramsden, Biopolymers 46 (7), 489 (1998).
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © R.M. Sarimov, T.A. Matveyeva, A.L. Vasin, V.N. Binhi, 2017, published in Biofizika, 2017, Vol. 62, No. 2, pp. 243–248.
Rights and permissions
About this article
Cite this article
Sarimov, R.M., Matveyeva, T.A., Vasin, A.L. et al. Changes in the refractive index of a solution during proteolysis of bovine serum albumin with pepsin. BIOPHYSICS 62, 177–181 (2017). https://doi.org/10.1134/S0006350917020221
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0006350917020221