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Structural adaptation of active center channels of octaheme nitrite reductases from the haloalkaliphilic bacteria Thioalkalivibrio nitratireducens to a proton deficit

  • Molecular Biophysics
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Abstract

Study of the adaptation mechanisms of proteins from extremophiles paves the way for the development of new biocatalysts that are resistant to extreme conditions. Here, we studied the structural adaptation of active center channels of octaheme nitrite reductase from the haloalkophilic bacterium Thioalkalivibrio nitratireducens (TvNiR) to high pH. Comparative analysis of the structures of octaheme nitrite reductases adapted to different environmental conditions revealed unique adaptation mechanisms for TvNiR, which play an important role in binding rare protons and substrate and product migration in the active-site channels.

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Abbreviations

DSC:

differential scanning calorimetry

СD:

circular dichroism

ONR:

octaheme nitrite reductase

GsNiR:

ONR from non-halophilic bacterium Geobacter sulfurreducens

TvNiR:

ONR from haloalkaliphilic bacterium Thioalkalivibrio nitratireducens

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Authors and Affiliations

  1. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, 119071, Russia

    A. V. Popinako, T. V. Tikhonova & V. O. Popov

  2. Ammosov North-Eastern Federal University, Yakutsk, Republic of Sakha (Yakutia), 677980, Russia

    M. Yu. Antonov

  3. Moscow State University, Moscow, 119992, Russia

    K. V. Shaitan

Authors
  1. A. V. Popinako
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  2. T. V. Tikhonova
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  3. M. Yu. Antonov
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  4. K. V. Shaitan
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  5. V. O. Popov
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Corresponding author

Correspondence to A. V. Popinako.

Additional information

The article was translated by the authors.

Original Russian Text © A.V. Popinako, T.V. Tikhonova, M.Yu. Antonov, K.V. Shaitan, V.O. Popov, 2017, published in Biofizika, 2017, Vol. 62, No. 2, pp. 284–289.

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Popinako, A.V., Tikhonova, T.V., Antonov, M.Y. et al. Structural adaptation of active center channels of octaheme nitrite reductases from the haloalkaliphilic bacteria Thioalkalivibrio nitratireducens to a proton deficit. BIOPHYSICS 62, 214–219 (2017). https://doi.org/10.1134/S0006350917020191

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  • DOI: https://doi.org/10.1134/S0006350917020191

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