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Mitochondrial DNA deletions in the peripheral blood of workers at the Mayak PA who were exposed to long-term combined effects of external γ- and internal α-radiation

  • Complex Systems Biophysics
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Abstract

The levels of large deletions in the mitochondrial DNA of workers at the Mayak Production Association (Mayak PA) who were exposed to external and combined occupational (external γ- and internal α-rays) radiation during the course of their duties were investigated. Peripheral blood-derived DNA samples were provided by the Radiobiological Human Tissue Repository of the Southern Urals Biophysics Institute (Russia). The samples were analyzed using long-extension PCR. The number of large-scale deletions in the mitochondrial DNA of workers who, in addition to external γ-radiation, were exposed to extra doses of irradiation due to incorporated 239Pu with a Pu body burden of 0.77–4.32 kBq, was 2.5-times lower compared to that of individuals who received only external γ-radiation. No significant gender-associated effects on the number of mitochondrial DNA deletions were detected among age-matched individuals.

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Authors and Affiliations

  1. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, ul. Institutskaya 3, Pushchino, Moscow oblast, 142290, Russia

    L. V. Malakhova, M. G. Lomaeva, V. N. Antipova & V. G. Bezlepkin

  2. Southern Urals Biophysics Institute, Federal Medicobiological Agency, sh. Ozyorskoe 19, Ozyorsk, Chelyabinsk oblast, 456780, Russia

    M. L. Zakharova, E. N. Kirillova & S. N. Sokolova

Authors
  1. L. V. Malakhova
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  2. M. G. Lomaeva
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  3. M. L. Zakharova
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  4. E. N. Kirillova
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  5. S. N. Sokolova
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  6. V. N. Antipova
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  7. V. G. Bezlepkin
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Corresponding authors

Correspondence to M. L. Zakharova or V. G. Bezlepkin.

Additional information

Original Russian Text © L.V. Malakhova, M.G. Lomaeva, M.L. Zakharova, E.N. Kirillova, S.N. Sokolova, V.N. Antipova, V.G. Bezlepkin, 2016, published in Biofizika, 2016, Vol. 61, No. 6, pp. 1236–1242.

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Malakhova, L.V., Lomaeva, M.G., Zakharova, M.L. et al. Mitochondrial DNA deletions in the peripheral blood of workers at the Mayak PA who were exposed to long-term combined effects of external γ- and internal α-radiation. BIOPHYSICS 61, 1026–1032 (2016). https://doi.org/10.1134/S0006350916060142

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  • DOI: https://doi.org/10.1134/S0006350916060142

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