Abstract
We have developed a technique for the immobilization of inulinase on chitosans with different molecular weights. The acid-soluble mid-molecular-weight (200 kDa) and high-molecular-weight (350 kDa) chitosans are shown to be promising matrices for inulinase adsorption. We assumed that the formation of an inulinase-chitosan matrix complex occurs mainly due to hydrophobic interactions; electrostatic interactions also play an essential role. The enzyme complex with high-molecular-weight chitosan is more stable against the action of UV light and temperature. This allows us to recommend chitosan as a catalyst in industry for the production of fructose from inulin-containing vegetable raw mateials.
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Original Russian Text © M.G. Holyavka, V.G. Artyukhov, P.V. Seredin, 2016, published in Biofizika, 2016, Vol. 61, No. 6, pp. 1109–1117.
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Holyavka, M.G., Artyukhov, V.G. & Seredin, P.V. The interaction of inulinase molecules with a chitosan matrix: UV-induced changes in the functional properties of immobilized inulinase. BIOPHYSICS 61, 871–876 (2016). https://doi.org/10.1134/S0006350916060099
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DOI: https://doi.org/10.1134/S0006350916060099