Abstract
NMR proton spectra were recorded in the range of proton resonance in the nucleotide aromatic ring of monomeric ATP–G-actin and the Mg2+–ATP–G-actin solutions in D2O to study the mechanism of ATP–G-actin hydrolysis and its role in F-actin formation in Mg2+-containing solutions. The experimental data show variations in the proton chemical shifts of the H2 and H8 peaks and splitting of the H8 resonance peak of G-actin-bound ATP adenine caused by interaction with magnesium dication. The observed variations in spectra are explained by hydrolysis of monomeric ATP–G-actin to ADP–G-actin, which is regarded as the initial stage of the G-actin to F-actin transformation.
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Original Russian Text © V.N. Umetskaya, 2016, published in Biofizika, 2016, Vol. 61, No. 4, pp. 692–698.
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Umetskaya, V.N. The mechanism of ATP–G-actin hydrolysis in Mg2+-containing solutions. BIOPHYSICS 61, 585–590 (2016). https://doi.org/10.1134/S0006350916040242
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DOI: https://doi.org/10.1134/S0006350916040242