Abstract
Using Raman and infrared spectroscopy it has been found that during the normal life of algae (pH changes from 8.0 to 9.0) the content of carotenoids increases and the molecules change their conformation: the contribution of–C=C–bonds of the polyene chain of a carotenoid molecule (Raman spectroscopy) is reduced and the contribution of methyl groups (∼2940 cm–1) and aromatic C–H-plane deformation vibrations (band at 1050 cm–1) of carotenoid molecules (infrared spectroscopy) decreases as well. It is the opinion of the authors that a change in the extracellular pH within the normal range has no influence on the content of chlorophyll a and b, but tends to increase the content and alter the conformation or structure of carotenoid molecules.
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Abbreviations
- RS:
-
Raman scattering
- IR:
-
infrared
References
Y. Yoshii, T. Hanyuda, I. Wakana, et al., J. Phycol. 40, 1170 (2004).
P. Jahns and A. R. Holzwarth, Biochim. Biophys. Acta 1817, 182 (2012).
N. P. Kutuzov, A. R. Brazhe, G. V. Maksimov, et al., Biophys. J. 107 (4), 891 (2014).
P. Jahns, D. Latowski, and K. Strzalka, Biochim. Biophys. Acta 1787, 3 (2009).
E. G. Maksimov, E. A. Shirshin, N. N. Sluchanko, et al., Biophys. J. 109 (3), 595 (2015).
S. Takaichiemail, Mar. Drugs 9 (6), 1101 (2011).
Koyama Y. and R. Fuji, in Photochemistry of Carotenoids, Ed. by H. A. Frank, A. J. Young, G. Britton and R. J. Cogdell (Kluwer, 2004), pp. 161–188.
B. Robert, in Photochemistry of Carotenoids, Ed. by H. A. Frank, A. J. Young, G. Britton and R. J. Cogdell (Kluwer, 2004), pp. 189–201.
W.-L. Liu, Zh.-G. Wang, Zh.-R. Zheng, et al., J. Phys. Chem. A 11, 10580 (2008).
A. Andreeva, V. Apostolova, and M. Velitchkova, Spectrochim. Acta A: Biomol. Spectrosc. 78, 1261 (2011).
W.I. Gruszecki, M. Gospodarek, W. Grudzinski, et al., J. Phys. Chem. B 113, 2506 (2009).
B. Bethmann and G. Schönknecht, New Phytol. 183 (2), 327 (2009).
M. Denesh, V. K. Andrianov, A. A. Bulychev, et al., Biochim. Biophys. Acta 1325 (1), 63 (1997).
A. Katz, M. Bental, H. Degani, et al., 96 (1), 110 (1991).
A. N. Zhuravskaya, I. V. Voronov, and E. R. Poskachina, Nauka Obraz. No. 4, 65 (2011).
C. N. Radenovic, G. V. Maksimov, E. V. Tyutyaev, et al., in Plant Physiology and Biochemistry (Elsevier, 2014), Vol. 81, pp. 208–211.
G. V. Maksimov, C. N. Radenovich, Yu. E. Borisova, et al., Biofizika 41, 400 (1996).
C. Ilioaia, M. P. Johnson, C. D. P. Duffy, et al., J. Biol. Chem. 286 (1), 91 (2011).
D. W. Brown, A. J. Floyd, and M. J. Sainsbury, Organic Spectroscopy (Wiley, New York, 1988; Mir, Moscow, 1992).
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Original Russian Text © V.V. Shutova, E.V. Tyutyaev, A.A. Churin, V.Yu. Ponomarev, G.A. Belyakova, G.V. Maksimov, 2016, published in Biofizika, 2016, Vol. 61, No. 4, pp. 711–716.
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Shutova, V.V., Tyutyaev, E.V., Churin, A.A. et al. IR and Raman spectroscopy in the study of carotenoids of Cladophora rivularis algae. BIOPHYSICS 61, 601–605 (2016). https://doi.org/10.1134/S0006350916040217
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DOI: https://doi.org/10.1134/S0006350916040217