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Biophysics

, Volume 61, Issue 3, pp 420–428 | Cite as

The effects of antioxidants and hypohalous acid scavengers on neutrophil activation by hypochlorous acid-modified low-density lipoproteins

  • O. M. PanasenkoEmail author
  • E. V. Mikhalchik
  • I. V. Gorudko
  • D. V. Grigorieva
  • A. V. Sokolov
  • V. A. Kostevich
  • V. B. Vasilyev
  • S. N. Cherenkevich
Cell Biophysics

Abstract

Hypochlorous acid-modified human blood low density lipoprotein (LDL–HOCl) was shown to stimulate neutrophils and to increase the luminol- (lm-CL) or lucigenin-enhanced chemiluminescence (lc-CL) of neutrophils. Antioxidants and HOCl scavengers (glutathione, taurine, cysteine, methionine, ceruloplasmin, and human serum albumin (HSA)) were tested for effects on lm-CL, lc-CL, H2O2 production, and degranulation of azurophilic granules of neutrophils. All agents used in increasing concentrations were found to decrease lm-CL produced by neutrophils upon stimulation with LDL–HOCl or subsequent treatment with the activator phorbol 12-myristate 13-acetate (PMA). The agents exerted a far lower, if any, effect on lc-CL and the H2O2 production by neutrophils in the same conditions. In the majority of cases, a decline in neutrophil chemiluminescence in the presence of the agents was not related to their effect on neutrophil degranulation, but was most likely due to their direct interactions with reactive halogen (RHS) or oxygen (ROS) species generated upon neutrophil activation or to myeloperoxidase (MPO) inhibition. Antioxidants and HOCl scavengers present in the human body were assumed to decelerate the development of oxidative or halogenative stress and thereby prevent neutrophil activation.

Keywords

halogenative stress oxidative stress low density lipoprotein neutrophil activation reactive halogen species chemiluminescence 

Abbreviations

MPO

myeloperoxidase

RHS

reactive halogen species

HSA

human serum albumin

LDL

low density lipoprotein

ROS

reactive oxygen species

PMA

phorbol 12-myristate 13-acetate

fMLP

N-formyl-Met-Leu-Phe

PBS

phosphate-buffered saline

LDL–HOCl

HOCl-modified LDL

lm-CL

luminol-enhanced chemiluminescence

lc-CL

lucigenin-enhanced chemiluminescence

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Copyright information

© Pleiades Publishing, Inc. 2016

Authors and Affiliations

  • O. M. Panasenko
    • 1
    • 2
    Email author
  • E. V. Mikhalchik
    • 1
  • I. V. Gorudko
    • 3
  • D. V. Grigorieva
    • 3
  • A. V. Sokolov
    • 1
    • 4
    • 5
  • V. A. Kostevich
    • 1
    • 4
  • V. B. Vasilyev
    • 4
    • 5
  • S. N. Cherenkevich
    • 3
  1. 1.Research Institute of Physico-chemical MedicineFederal Medico-Biological Agency of RussiaMoscowRussia
  2. 2.Ministry of Health and Social Development of the Russian FederationPirogov Russian National Research Medical UniversityMoscowRussia
  3. 3.Belarussian State UniversityMinskBelarus
  4. 4.Institute of Experimental MedicineSt. PetersburgRussia
  5. 5.St. Petersburg State UniversitySt. PetersburgRussia

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