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Decomposition of the hemoglobin UV absorption spectrum into the absorption spectra of a prosthetic group and apoprotein using an additive model

  • Molecular Biophysics
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Abstract

The decomposition pathways of the hemoglobin UV-absorption spectrum into the absorption spectra of the protein and non-protein components were proposed and substantiated by means of an additive model. We have established that the heme component has an absorption band with a maximum at λmax = 269.2 nm (ε = 97163) and the apoprotein component has an absorption band with a maximum at λmax = 278.4 nm (ε = 48669) for the wavelength range from 240.0 to 320.0 nm. The integral relative proportion of absorption for the heme fraction (78.8%) and apoprotein (21.2%) in the investigating wave-length range was determined.

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Authors and Affiliations

  1. Voronezh State University, Voronezh, 394006, Russia

    I. A. Lavrinenko, G. A. Vashanov & V. G. Artyukhov

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  1. I. A. Lavrinenko
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  2. G. A. Vashanov
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  3. V. G. Artyukhov
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Correspondence to I. A. Lavrinenko.

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Original Russian Text © I.A. Lavrinenko, G.A. Vashanov, V.G. Artyukhov, 2015, published in Biofizika, 2015, Vol. 60, No. 2, pp. 253–261.

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Lavrinenko, I.A., Vashanov, G.A. & Artyukhov, V.G. Decomposition of the hemoglobin UV absorption spectrum into the absorption spectra of a prosthetic group and apoprotein using an additive model. BIOPHYSICS 60, 197–204 (2015). https://doi.org/10.1134/S0006350915020098

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  • DOI: https://doi.org/10.1134/S0006350915020098

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