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The process of heme synthesis in bone marrow mesenchymal stem cells cultured with fibroblast growth factor bFGF and under hypoxic conditions

  • Cell Biophysics
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Abstract

It was shown that fibroblast growth factor bFGF influences the process of heme synthesis and the proliferative activity and viability of bone marrow mesenchymal stem cells in culture under hypoxic conditions. The addition of fibroblast growth factor bFGF (7 ng/mL) to the medium under the above conditions led to the accumulation of aminolevulinic acid (an early porphyrin and heme precursor), an increase in the expression of CD71 (transferrin receptor), and a decrease in the content of porphyrin pigments and heme (a late precursor and end product of heme synthesis, respectively). It was found that cultivation of the cells under hypoxic conditions and bFGF is optimal for maintaining high viability and proliferation capacity of the mesenchymal stem cells.

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Abbreviations

MSCs:

mesenchymal stem cells

bFGF:

fibroblast growth factor b

ALA:

aminolevulinic acid

CD71:

transferrin receptor protein

FITC:

fluorescein isothiocyanate

FDA:

fluorescein diacetate

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Authors and Affiliations

  1. Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, ul. Akademicheskaya 27, Minsk, 220073, Belarus

    A. G. Poleshko, E. S. Lobanok & I. D. Volotovski

  2. Institute of Cell Biophysics, Russian Academy of Sciences, ul. Institutskaya 3, Pushchino, Moscow oblast, 142290, Russia

    L. M. Mezhevikina & E. E. Fesenko

Authors
  1. A. G. Poleshko
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  2. E. S. Lobanok
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  3. L. M. Mezhevikina
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  4. E. E. Fesenko
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  5. I. D. Volotovski
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Correspondence to A. G. Poleshko.

Additional information

Original Russian Text © A.G. Poleshko, E.S. Lobanok, L.M. Mezhevikina, E.E. Fesenko, I.D. Volotovski, 2014, published in Biofizika, 2014, Vol. 59, No. 6, pp. 1125–1130.

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Poleshko, A.G., Lobanok, E.S., Mezhevikina, L.M. et al. The process of heme synthesis in bone marrow mesenchymal stem cells cultured with fibroblast growth factor bFGF and under hypoxic conditions. BIOPHYSICS 59, 913–917 (2014). https://doi.org/10.1134/S0006350914060177

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  • DOI: https://doi.org/10.1134/S0006350914060177

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