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Specific properties of the mitochondrial oxidative phosphorylation system operating as a supercomplex

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Abstract

This paper presents a study of endogenous and exogenous fatty acids affecting the efficiency of the mitochondrial phosphorylation system (the ADP/O parameter) depending on temperature. The experiment was carried out under conditions in which the oxidative phosphorylation system operated as a supercomplex. Rat liver mitochondria were isolated without deliberate removal of fatty acids from membranes and studied then in hypotonic medium (120 mOsm). We managed to detect a very narrow interval (19 ± 1°C) where the fatty acid uncoupling effect is weak up to the point of vanishing. In the same small temperature range, a structural rearrangement, which takes place in the enzyme system, is accompanied with a denser packing of membrane protein complexes. Thus, at temperatures close to 19°C, the supercomplex works in the specific regime protected (or partially protected) from the uncoupling effect of fatty acids. Here, we also discuss the physiological significance of the increased efficiency of ATP synthesis at lower temperatures. We present the most probable character of the structural rearrangement in the enzyme system of mitochondrial membrane at 19°C.

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Authors and Affiliations

  1. Moscow Institute of Physics and Technology, Institutskii per. 9, Dolgoprudny, Moscow oblast, 141700, Russia

    S. V. Nesterov, Y. A. Skorobogatova & L. S. Yaguzhinsky

  2. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, 119991, Russia

    S. V. Nesterov, Y. A. Skorobogatova & L. S. Yaguzhinsky

Authors
  1. S. V. Nesterov
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  2. Y. A. Skorobogatova
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  3. L. S. Yaguzhinsky
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Correspondence to L. S. Yaguzhinsky.

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Original Russian Text © S.V. Nesterov, Y.A. Skorobogatova, L.S. Yaguzhinsky, 2014, published in Biofizika, 2014, Vol. 59, No. 6, pp. 1113–1120.

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Nesterov, S.V., Skorobogatova, Y.A. & Yaguzhinsky, L.S. Specific properties of the mitochondrial oxidative phosphorylation system operating as a supercomplex. BIOPHYSICS 59, 904–909 (2014). https://doi.org/10.1134/S0006350914060141

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  • DOI: https://doi.org/10.1134/S0006350914060141

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