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Specific molecular and morphological changes in cardiomyocytes of hibernating ground squirrels in different periods of annual cycle

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Abstract

Structural and molecular changes in cardiomyocytes of hibernating ground squirrels in different periods of the annual cycle were analyzed by means of electron microscopy and polymerase chain reaction. Morphological analysis showed an increase in relative area of sarcoplasmic reticulum in cardiac muscle of ground squirrels preparing to torpor compared to active summer animals. The size of sarcoplasmic reticulum in cardiomyocytes of torpid animals was reliably less than in any other condition of ground squirrels in the annual cycle. The results of molecular analysis showed the decrease in sarcoplasmic reticulum Ca2+-ATPase gene (SERCA2a) expression at all stages of hibernation process and also in periods of autumn activity compared to control mRNA level in active summer animals. The revealed season changes in structure of sarcoplasmic reticulum and sarcoplasmic reticulum Ca2+-ATPase gene expression are discussed in regard to adaptation of ground squirrels to hibernation.

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Authors and Affiliations

  1. Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia

    E. V. Karaduleva & N. M. Zakharova

  2. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia

    I. M. Santalova

Authors
  1. E. V. Karaduleva
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  2. I. M. Santalova
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  3. N. M. Zakharova
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Correspondence to E. V. Karaduleva.

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Original Russian Text © E.V. Karaduleva, I.M. Santalova, N.M. Zakharova, 2014, published in Biofizika, 2014, Vol. 59, No. 5, pp. 926–932.

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Karaduleva, E.V., Santalova, I.M. & Zakharova, N.M. Specific molecular and morphological changes in cardiomyocytes of hibernating ground squirrels in different periods of annual cycle. BIOPHYSICS 59, 752–757 (2014). https://doi.org/10.1134/S0006350914050091

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  • DOI: https://doi.org/10.1134/S0006350914050091

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