Abstract
The objective of the present research was to study transformations of various physiological NO forms in an isolated rat heart, perfused with the medium containing dinitrosyl iron complexes with glutathione ligand (DNIC-GH). We showed that such aerobic perfusion resulted in accumulation of mostly diamagnetic NO physiological forms (S-nitrosothiols) in myocardial tissue. They were transformed into protein-bound mononuclear dinitrosyl iron complexes during subsequent total ischemia. Meantime, DNIC-GH injection on the onset of ischemia resulted in changes in the state of mitochondrial respiratory chain, characterized by the increase in myocardial concentration of flavosemiquinones.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
A. F. Vanin, Nitric Oxide: Biol.Chem. 21, 1 (2009).
Y. S. Rahmanto, D. S. Kalinowski, D. J. R. Lane, et al., J. Biol. Chem. 287(10), 6960 (2012).
A. F. Vanin, A. P. Poltorakov, V. D. Mikoyan, et al., Nitric Oxide: Biol. Chem. 23, 136 (2010).
V. L. Lakomkin, A. F. Vanin, A. A. Timoshin, et al., Nitric Oxide: Biol. Chem. 16(4), 413 (2007).
O. I. Pisarenko, L. I. Serebryakova, O. V. Tskitishvili, et al., Izv. RAN, Ser. Biol., no. 1, 110 (2008).
A. A. Timoshin, O. I. Pisarenko, O. V. Tskitishvili, et al., Biophysics 55(6), 999 (2010).
E. I. Chazov, O. V. Rodnenkov, A. V. Zorin, et al., Kardiologiya 51(11), 39 (2011).
E. I. Chazov, O. V. Rodnenkov, A. V. Zorin, et al., Nitric Oxide: Biol. Chem. 26(3), 148 (2012).
O. I. Pisarenko, V. S. Shulzhenko, I. M. Studneva, et al., Biophysics 54(6), 709 (2009).
A. F. Vanin, V. O. Lozinskii, and V. I. Kapel’ko, RF Patent 2291880 (2005).
A. F. Vanin and E. van Faassen, in Radicals for Live: The Various Forms of Nitric Oxide, by Eds. E. E. van Faassen, A. F. Vanin (Elsevier, Amsterdam, 2007), pp. 19–74.
L.-L. Wang, Z. Guo, Y. Han, et al., Regulatory Peptides 167(2–3), 185 (2011).
G. Backstrom, B. Norling, A. Ehrenberg, and L. Ernster, Biochim. Biophys. Acta 197(1), 108 (1970).
E. K. Ruuge, K. P. Kashkarov, V. L. Lakomkin, et al., Mol. Aspects Med. 18(Suppl.), s41 (1997).
G. Palmer, F. Muller, P. Hemmerich, et al., Eur. J. Biochem. 14(1), 185 (1970).
A. A. Timoshin, O. I. Pisarenko, V. L. Lakomkin, et al., Exp. Clin. Cardiology 5(2), 59 (2000).
A. Webb, R. Bond, P. McLean, et al., Proc. Natl. Acad. Sci. USA 101, 13683 (2004).
M. T. Gladwin, N. J. H. Raat, S. Shiva, et al., Am. J. Physiol. Heart. Circ. Physiol. 291, H2026 (2006).
K. B. Shumaev, A. A. Gubkin, V. A. Serezhenkov, et al., Nitric Oxide: Biol. Chem. 18, 37 (2008).
A. A. Timoshin, D. Yu. Drobotova, O. V. Tskitishvili, et al., Dokl. RAN 432(3), 416 (2010).
Author information
Authors and Affiliations
Additional information
Original Russian Text © A.A. Timoshin, V.L. Lakomkin, D.Yu. Drobotova, E.K. Ruuge, A.F. Vanin, 2013, published in Biofizika, 2013, Vol. 58, No. 2, pp. 281–288.
Rights and permissions
About this article
Cite this article
Timoshin, A.A., Lakomkin, V.L., Drobotova, D.Y. et al. Transformations of dinitrosyl iron complexes in an isolated rat heart after introduction of this substance into perfusion medium. BIOPHYSICS 58, 206–211 (2013). https://doi.org/10.1134/S0006350913020218
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0006350913020218