Abstract
Formation of reactive oxygen species (ROS) during all-trans-retinal (ATR) illumination was studied by EPR. The quantity of ROS was estimated from the EPR signal of a spin adduct of DEPMPO (5-diethoxyphosphoryl-5-methyl-1-pyrroline-N-oxide). The EPR signal was found to be a superposition of the adducts: 80% superoxide anion radical, 15% hydroxyl radical, and 5% unknown radical. Albumin at an equimolar concentration decreased the relative quantum yield of ROS five times, and a 4-fold albumin excess decreased ROS production 30 times. It is supposed that ATR and A2E in the photoreceptor membrane are the sources of photodamage induced by ROS. Since ATR in the cell is known to be transported by interphotoreceptor retinal-binding proteins of the albumin family, its binding to protein is supposed to play an important protective role, preventing ROS production in the photoreceptor cell.
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Original Russian Text © P.V. Aboltin, T.F. Shevchenko, K.B. Shumaev, G.R. Kalamkarov, 2013, published in Biofizika, 2013, Vol. 58, No. 2, pp. 246–251.
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Aboltin, P.V., Shevchenko, T.F., Shumaev, K.B. et al. Photoinduced production of reactive oxygen species by retinal derivatives and conjugates. BIOPHYSICS 58, 178–182 (2013). https://doi.org/10.1134/S0006350913020024
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DOI: https://doi.org/10.1134/S0006350913020024