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Suppression of vascular endothelium hyperpermeability by cell-permeating peptide inhibitors of myosin light chain kinase

  • Molecular Biophysics
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Abstract

Novel peptides originating from the peptide inhibitor of myosin light chain kinase (MLCK), L-PIK (Arg-Lys-Lys-Tyr-Lys-Tyr-Arg-Arg-Lys), have been studied for their ability to attenuate the thrombin-induced hyperpermeability of an endothelial cell monolayer in culture. Peptides [NαMeArg1]-Lys-Lys-Tyr-Lys-Tyr-Arg-(D)Arg8-Lys and H-Arg(NO2)Lys-Lys-Tyr-Lys-Tyr-Arg-Arg-Lys-NH2 (designated PIK2 and PIK4, respectively) appeared to be the most effective inhibitors of endothelial cell monolayer hyperpermeability, and surpassed other known peptide inhibitors of MLCK derived from original L-PIK. Our results validate PIK2 and PIK4 as the leading molecules for the development of novel drugs intended to counteract pathological hyperpermeability of vascular endothelium.

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Authors and Affiliations

  1. Russian Cardiology Research and Production Center, Moscow, 121552, Russia

    A. Y. Khapchaev, M. V. Samsonov, O. A. Kazakova, E. L. Vilitkevich, M. V. Sidorova, A. A. Az’muko, A. S. Molokoedov, Zh. D. Bespalova & V. P. Shirinsky

Authors
  1. A. Y. Khapchaev
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  2. M. V. Samsonov
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  3. O. A. Kazakova
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  4. E. L. Vilitkevich
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  5. M. V. Sidorova
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  6. A. A. Az’muko
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  7. A. S. Molokoedov
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  8. Zh. D. Bespalova
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  9. V. P. Shirinsky
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Corresponding author

Correspondence to V. P. Shirinsky.

Additional information

Original Russian Text © A.Y. Khapchaev, M.V. Samsonov, O.A. Kazakova, E.L. Vilitkevich, M.V. Sidorova, A.A. Az’muko, A.S. Molokoedov, Zh.D. Bespalova, V.P. Shirinsky, 2012, published in Biofizika, 2012, Vol. 57, No. 5, pp. 764–770.

Translation of the text provided by the authors; redaction imposed solely for consistency and comprehensibility.

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Khapchaev, A.Y., Samsonov, M.V., Kazakova, O.A. et al. Suppression of vascular endothelium hyperpermeability by cell-permeating peptide inhibitors of myosin light chain kinase. BIOPHYSICS 57, 587–591 (2012). https://doi.org/10.1134/S0006350912050089

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  • DOI: https://doi.org/10.1134/S0006350912050089

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