Abstract
Structural and functional changes in Mongolian gerbil soleus fibers were analyzed after a 31-day hindlimb suspension. Contractile properties of muscle fibers were studied by means of tensometry; the transversal stiffness of different parts of the contractile apparatus was measured by atomic force microscopy, resting calcium level was estimated by fluorescence microscopy by using Fluo-4-AM; cytoskeletal protein content was determined by western blotting. It was shown that after gravitational unloading the maximal force of contraction and specific tension of fiber were significantly reduced, as well as calcium sensitivity actually lowered. At the same time, the transversal stiffness of Z-disk in the relaxed and activated state was decreased significantly compared to the control group. Desmin content was at the control level, but alpha-actinin-2, main structural protein of Z-disk, became considerably less after a 31-day hindlimb suspension. Besides, resting calcium level remained at control values during the simulated gravitational unloading. The data suggest that Z-disk destruction, as a result of alpha-actinin-2 content reduction, leads to changes in the lattice spacing and decreases contractile properties.
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L. Stevens, X. Holy, and Y. Mounier, Am. J. Physiol. 264, 770 (1993).
J. J. Widrick, J. G. Romatowski, K. M. Norenberg, et al., J. Appl. Physiol. 90, 2203 (2001).
E. V. Ponomareva, V. V. Kravtsova, E. V. Kachaeva, et al., Biofizika 53, 1087 (2008).
F. W. Booth and J. R. Kelso, Pflugers Arch. 342, 231 (1973).
D. Deplanches, M. H. Mayet, E. I. Ilyina-Kakueva, et al., J. Appl. Physiol. 68, 48 (1990).
V. J. Caiozzo, F. Haddad, M. J. Baker, et al., J. Appl. Physiol. 81, 123 (1996).
C. P. Ingalls, J. C. Wenke, and R. B. Armstrong, Aviat. Space Environ. Med. 75(5), 471 (2001).
I. V. Ogneva, V. A. Kurushin, E. G. Altaeva, et al., J. Muscle Res. Cell Motil. 30(7–8), 261 (2009).
M. Bartoli and I. Richard, Int. J. Biochem. Cell Biol. 37, 2115 (2005)
D. L. Enns, T. Raastad, I. Ugelstad, et al., Eur. J. Appl. Physiol. 100(4), 445 (2007).
K. S. McDonald and R. H. Fitts, J. Appl. Physiol. 79, 1796 (1995).
T. Toursel, L. Stevens, H. Granzier, et al., J. Appl. Physiol. 92, 1465 (2002).
V. J. Caiozzo, H. Richmond, S. Kaska, et al., J. Appl. Physiol. 103(4), 1150 (2007).
I. V. Ogneva, V. A. Kurushin, M. M. Glashev, et al., Biofizika 55, 1117 (2010).
E. N. Lipets, E. V. Ponomareva, I. V. Ogneva, et al., Aviakosm. Ekol. Med. 43(3), 34 (2009).
E. Morey-Holton, R. K. Globus, A. Kaplansky, et al., Adv. Space. Biol. Med. 10, 7 (2005).
I. V. Ogneva, D. V. Lebedev, and B. S. Shenkman, Biophys. J. 98(3) 418 (2010).
E. G. Altaeva, I. V. Ogneva, and B. S. Shenkman, Tsitologiya 52, 770 (2010).
I. V. Ogneva, J. Appl. Physiol. 109(6), (2010).
H. Towbin, T. Staehlin, and J. Gordon, Proc. Natl. Acad. Sci. USA 76, 4350 (1970).
Y. P. Wang and F. Fuchs, J. Muscle Res. Cell Motil. 21, 313 (2000).
D. Goll, V. Thompson, H. Li, et al., Physiol. Rev. 83, 731 (2003).
E. G. Altaeva, L. A. Lysenko, N. P. Kantserova, et al., Dokl. RAN 433(1), 138 (2010).
D. G. Peters, H. Mitchell-Felton, and S. C. Kandarian, Amer. J. Physiol. 276, 1218 (1999).
B. S. Shenkman, A. M. Moukhina, K. C. Litvinova, et al., J. Gravit. Physiol. 12, 119 (2005).
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Original Russian Text © E.V. Ponomareva, I.V. Ogneva, 2012, published in Biofizika, 2012, Vol. 57, No. 4, pp. 683–689.
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Ponomareva, E.V., Ogneva, I.V. Contractile properties, transversal stiffness and cytoskeletal protein content in Mongolian gerbils soleus fibers under long-term hindlimb suspension. BIOPHYSICS 57, 519–524 (2012). https://doi.org/10.1134/S0006350912040148
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DOI: https://doi.org/10.1134/S0006350912040148