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New possibilities of studying microbial objects by laser interference microscopy

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Abstract

A novel method, laser interference microscopy, has been developed for studying the morphofunctional state of bacterial cells and the structure of bacterial communities. The following potentialities of the method are shown: rapid determination of the cell structure and subcellular structures (nucleus zone, vacuoles, lamellar structures) and the physiological state of the cell, as well as the study of the structure of bacterial communities (biofilm). The method does not require any additional preparation of cells before the investigation (fixation, staining, treatment with contrasting substances), which reduces the possible appearance of artifacts to a minimum and enables one to use laser interference microscopy for in vivo investigations.

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Authors and Affiliations

  1. Vinogradskii Institute of Microbiology, Russian Academy of Sciences, Moscow, 117312, Russia

    Yu. Yu. Berestovskaya & L. M. Gerasimenko

  2. Biological Faculty, Moscow State University, Moscow, 119899, Russia

    A. I. Yusipovich, G. V. Maksimov & A. B. Rubin

  3. All-Russia Research Institute of Optical and Physical Measurements, Moscow, 119361, Russia

    G. G. Levin

  4. Biological Faculty, Mordovian State University, Saransk, 430000, Russia

    V. V. Shutova

Authors
  1. A. I. Yusipovich
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  2. Yu. Yu. Berestovskaya
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  3. V. V. Shutova
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  4. G. G. Levin
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  5. L. M. Gerasimenko
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  6. G. V. Maksimov
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  7. A. B. Rubin
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Additional information

Original Russian Text © A.I. Yusipovich, Yu.Yu. Berestovskaya, V.V. Shutova, G.G. Levin, L.M. Gerasimenko, G.V. Maksimov, A.B. Rubin, 2011, published in Biofizika, 2011, Vol. 56, No. 6, pp. 1091–1098.

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Yusipovich, A.I., Berestovskaya, Y.Y., Shutova, V.V. et al. New possibilities of studying microbial objects by laser interference microscopy. BIOPHYSICS 56, 1063–1068 (2011). https://doi.org/10.1134/S0006350911060224

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  • DOI: https://doi.org/10.1134/S0006350911060224

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