Abstract
A method has been elaborated that preserves the mitochondrial-reticular network in lymphocytes by immobilization of a blood smear on glass and its subsequent incubation in a medium close in composition to the physiological one. Physiological responses of mitochondrial respiration to excitation in the organism are well pronounced on these preparations. Detection of early responses of mitochondria to pathogenic agents in the organism is a topical problem of basic and medical research, since such alterations play a leading role in the development of pathological states.
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Original Russian Text © M.V. Zakharchenko, N.V. Khunderyakova, M.N. Kondrashova, 2011, published in Biofizika, 2011, Vol. 56, No. 5, pp. 840–847.
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Zakharchenko, M.V., Khunderyakova, N.V. & Kondrashova, M.N. Importance of preserving the biophysical organization of isolated mitochondria for revealing their physiological regulation. BIOPHYSICS 56, 810–815 (2011). https://doi.org/10.1134/S0006350911050290
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DOI: https://doi.org/10.1134/S0006350911050290