Abstract
A method is proposed for obtaining a free complex of transducin βγ subunits from bovine retinal rod outer segments in a highly purified state.
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L. Stryer, Annu. Rev. Neurosci. 9, 87 (1986).
L. Stryer, J. Biol. Chem. 266, 10711 (1991).
E. N. Pugh, Jr. and T. D. Lamb, Biochim. Biophys. Acta 1141(2–3), 111 (1993).
V. Yu. Arshavsky, T. D. Lamb, and E. N. Pugh., Jr., Annu. Rev. Physiol. 64, 153 (2002).
E. E. Fesenko, S. S. Kolesnikov, and A. L. Lyubarsky, Nature 313(6000), 310 (1985).
H. Kuhn, Curr. Topics Membr Transp. 15, 171 (1981).
J. W. Clack, L. Matthew, M. L. Springmeyer, et al., Cell Biol. Internat. 30, 829 (2006).
T. Wieland, B. Nurnberg, I. Ulibarri, et al., J. Biol. Chem. 268, 18111 (1993).
J. F. Klinker and R. Seifert, Eur. J. Biochem. 261, 72 (1999).
F. Cuello, R. A. Schulze, F. Heemeyer, et al., J. Biol. Chem. 278, 7220 (2003).
D. N. Orlov and N. Ya. Orlov, Biofizika 53, 922 (2008).
N. Ya. Orlov, E. V. Kalinin, T. G. Orlova, and A. A. Freidin, Biochim. Biophys. Acta 954, 325 (1988).
N. Ya. Orlov, T. G. Orlova, K. Nomura, et al., FEBS Lett. 389, 186 (1996).
U. K. Laemmli, Nature 227(5259), 680 (1970).
M. A. Bradford, Anal. Biochem. 72, 248 (1976).
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Original Russian Text © D.N. Orlov, T.G. Orlova, A.R. Nezvetsky, N.Ya. Orlov, 2010, published in Biofizika, 2010, Vol. 55, No. 6, pp. 986–989.
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Orlov, D.N., Orlova, T.G., Nezvetsky, A.R. et al. Purification of a complex of transducin βγ subunits from isotonic extracts of bovine retinal rod outer segments. BIOPHYSICS 55, 908–910 (2010). https://doi.org/10.1134/S0006350910060035
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DOI: https://doi.org/10.1134/S0006350910060035