Abstract
The role of endogenous porphyrins in the effect of laser irradiation on the superoxide dismutase (SOD) activity of wound exudate and rat leukocyte activity has been studied on models of aseptic incised skin wounds. Wounds were irradiated with a He-Ne laser (632.8 nm, 1.5 J/cm2) on the 2nd, 3rd, and 4th days after the beginning of the experiment. Irradiation effects were evaluated by the SOD activity (NBT test) and the activity of leukocytes of the wound exudate (as a chemiluminescent response to opsonized zymosan). It was found that in animals subjected to laser irradiation, the SOD activity sharply increased. This effect depended on endogenous porphyrin concentration and was retained throughout the experiment. The SOD activity in unirradiated animals decreased from the 2nd to the 5th day of experiment. The evaluation of the activity of wound exudate leukocytes did not reveal any distinct dependence of the effect on the concentration of endogenous porphyrins.
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Abbreviations
- CL:
-
chemiluminescence
- SOD:
-
superoxide dismutase
- PP:
-
protoporphyrin
References
A. Ekima, O. Armagana, F. Tascioglua, et al., Swiss Med. Wkly 137, 347 (2007).
V. T. Pal’chun, A. S. Lapchenko, and A. G. Kucherov, Lechebnoe Delo no. 2, 20 (2005).
B. T. Ivandic and T. Ivandic, Photomed. Laser Surg. 26(3), 241 (2008).
T. S. Alster and E. L. Tanzi, Dermatol. Surg. 35(3), 483 (2009).
A. Sugar, C. J. Rapuano, W. W. Culbertson, et al., Ophthalmology 109(1), 175 (2002).
B. Greve and C. Raulin, Dermatol. Surg. 28(2), 156 (2002).
O. Ozcelik, M. Cenk Haytac, A. Kunin, and G. Seydaoglu, J. Clin. Periodontol. 35(3), 250 (2008).
A. E. Saltmarche, Int. Wound J. 5(2), 351 (2008).
R. Mittermayr, A. Osipov, C. Piskernik, et al., Mol. Med. 13(1–2), 22 (2007).
G. I. Klebanov, T. V. Chichuk, A. N. Osipov, and Yu. A. Vladimirov, Biofizika 50(4), 713 (2005).
G. I. Klebanov, I. A. Strashkevich, T. V. Chichuk, et al., Biol. Membrany 15(3), 273 (1998).
Yu. A. Vladimirov, Three Hypotheses on the Mechanism of Action of Laser Radiation on Human Cells and Organism. Efferent Medicine (Inst. Biomed. Khimii RAMN, Moscow, 1994) [in Russian].
R. V. Gorenkov, V. N. Karpov, D. A. Rogatkin, and V. I. Shumskii, Biofizika 52(4), 711 (2007).
K. A. Askarov, B. D. Berezin, E. V. Bystritskaya, et al., Porphyrins: Spectroscopy, Electrochemistry, Application (Nauka, Moscow, 1987) [in Russian].
L. I. Slutskii, Biochemistry of Normal and Pathologically Altered Connective Tissue (Meditsina, L., 1969) [in Russian].
T. V. Machneva, D. M. Protopopov, Yu. A. Vladimirov, and A. N. Osipov, Biofizika 53(5), 894 (2008).
G. I. Klebanov, N. Yu. Shuraeva, T. V. Chichuk, et al., Biofizika 50(6), 1137 (2005).
A. Boyum, Scand. J. Clin. Invest. 21(97), 77 (1968).
G. P. Gurinovich, A. N. Sevchenko, and K. N. Solov’ev, Usp. Fiz. Nauk LXXIX(2), 173 (1963).
B. Halliwel and J. M. C. Gutteridge, Free Radicals in Biology and Medicine, 3nd ed. (Oxford University Press, Singapore, 2001).
M. I. Kuzin and B. M. Kostyuchenok, Wounds and Wound Infection. Manual for Physicians (Meditsina, Moscow, 1990) [in Russian].
G. I. Klebanov, N. Yu. Shuraeva, T. V. Chichuk, et al., Biofizika 51(1), 116 (2006).
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Original Russian Text © T.V. Machneva, N.N. Bulgakova, Yu.A. Vladimirov, A.N. Osipov, 2010, published in Biofizika, 2010, Vol. 55, No. 3, pp. 532–538.
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Machneva, T.V., Bulgakova, N.N., Vladimirov, Y.A. et al. The role of endogenous porphyrins in laser therapy of experimental skin wounds. BIOPHYSICS 55, 467–472 (2010). https://doi.org/10.1134/S000635091003019X
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DOI: https://doi.org/10.1134/S000635091003019X