Abstract
We introduced a novel approach for the establishment of genetically modified hESC lines, and have shown that mutant hESC may be derived from affected embryos after preimplantation genetic diagnosis (PGD) screening for a particular single gene disorder. Here we describe the procedure of embryo and cell manipulation, their diagnostic layout, and the analysis of the efficiency of embryo development and hESC establishment, as well as the developments for hESC derivation in animal-product-free conditions. Our study shows that a high efficiency of hESC derivation (50%) is especially crucial when working with rare and unique resources such as genetically screened embryos necessary for the derivation of hESC lines that represent specific genetic diseases.
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Original Russian Text © V. Galat, R.S. Ozen, Yu. Verlinsky, H. Greiss, E. Krotova, A. Mazepa, L.M. Chailakhyan, Ph. Iannaccone, 2010, published in Biofizika, 2010, Vol. 55, No. 3, pp. 481–485.
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Galat, V., Ozen, R.S., Verlinsky, Y. et al. Cell engineering and genetic approaches to development of human embryonic stem cell models for genetic disorders. BIOPHYSICS 55, 425–428 (2010). https://doi.org/10.1134/S0006350910030115
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DOI: https://doi.org/10.1134/S0006350910030115