Abstract
A study was made of the formation of ice microparticles (IMP) during freezing of cryoprotective media and the survival of fish sperm in cryopreservation. The IMP shape and size were determined by cryomicroscopy. It is shown that with an increase of the cooling rate the IMP area and perimeter decrease. Under ultrafast cooling (3000–4000 deg/min) in a thin layer (0.1 mm) the medium is vitrified. Additional components (egg yolk, sugars and lipids) substantially alter the shape and size of IMP. The intactness of sperm after cryopreservation does not always correlate with the IMP size and shape in the frozen cryoprotective solution. Formation of rounded particles with blurred boundaries correlates with sperm survival in cryopreservation.
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Original Russian Text © A.A. Andreev, D.G. Sadikova, E.N. Gakhova, T.N. Pashovkin, A.M. Tikhomirov, 2009, published in Biofizika, 2009, Vol. 54, No. 5, pp. 869–875.
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Andreev, A.A., Sadikova, D.G., Gakhova, E.N. et al. Congelation of cryoprotective solutions and cryopreservation of fish sperm. BIOPHYSICS 54, 612–616 (2009). https://doi.org/10.1134/S0006350909050108
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DOI: https://doi.org/10.1134/S0006350909050108