Abstract
Resonance light scattering (RLS), a phenomenon of abrupt enhancement of Rayleigh light scattering in close proximity to an absorption band, is easily detectable in solutions of strongly absorbing chromophores, which form large aggregates with strong π-electronic coupling among the chromophores. RLS spectra need to be corrected for the sensitivity of the spectrofluorimeter as well as for the effects of internal light filter. A method for correcting the measured RLS is described. It was shown by the method that addition of KCl induces formation of extended supramolecular aggregates (probably of H-type) of the anionic dye merocyanine 540 in water. The RLS spectra of a photosensitizer m-tetra(hydroxyphenyl)chlorin (Foscan®) indicate formation of J-aggregates of this dye in aqueous medium.
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Original Russian Text © A.M. Tikhomirov, T.A. Shmigol, E.A. Kozhinova, A.A. Kyagova, L.N. Bezdetnaya, A.Ya. Potapenko, 2009, published in Biofizika, 2009, Vol. 54, No. 5, pp. 824–830.
Editor’s Note: I certify that this text exactly reproduces all factual statements and closely conveys the phrasing and style of the original Russian publication. A.G.
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Tikhomirov, A.M., Shmigol, T.A., Kozhinova, E.A. et al. Correction of spectra for studying dye aggregates by resonance light scattering. BIOPHYSICS 54, 584–589 (2009). https://doi.org/10.1134/S0006350909050054
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DOI: https://doi.org/10.1134/S0006350909050054