Skip to main content
SpringerLink
Log in

Peculiarities of DNA-proflavine binding under different concentration ratios

  • Molecular Biophysics
  • Published:
Biophysics Aims and scope Submit manuscript

Abstract

Binding of proflavine to calf thymus DNA is investigated by differential scanning calorimetry and spectrophotometry. It is shown that proflavine interacts with DNA by three binding modes. At high DNA—ligand concentration ratios (P/D), proflavine prefers to intercalate into GC-sites but can also insert into other sites. At low P/D ratios, proflavine interacts with DNA by the external binding mode. The parameters of proflavine-DNA complexation have been calculated from spectrophotometric concentration dependences. Thermodynamic parameters of DNA melting have been calculated from differential scanning calorimetry data.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. L. R. Ferguson and W. A. Denny, Mutat. Res. 258(2), 123 (1991).

    Google Scholar 

  2. S. Georghiou, Photochem. Photobiol. 26(1), 59 (1977).

    Article  Google Scholar 

  3. S. Neidle, L. H. Pearl, P. Herzyk, and H. M. Bernan, Nucl. Acids Res. 16(18), 8999 (1988).

    Article  Google Scholar 

  4. L. S. Lerman, Proc. Nat. Acad. Sci. 49(1), 94 (1963).

    Article  ADS  Google Scholar 

  5. D. J. Patel, Biopolymers 16(12), 2739 (1977).

    Article  Google Scholar 

  6. C. Ciatto, M. L. D’Amico, G. Natile, and F. Secco, Biophys. J. 77(5), 2717 (1999).

    Article  Google Scholar 

  7. M. Dourlent and C. Helene, Eur. J. Biochem. 23(1), 86 (1971).

    Article  Google Scholar 

  8. Y. Kubota, K. Hirano, and Y. Motoda, Chem. Lett. 7(1), 123 (1978).

    Article  Google Scholar 

  9. V. Deubel and M. Leng, Biochimie 56, 641 (1974).

    Article  Google Scholar 

  10. A. T. Karapetian, P. O. Vardevanian, G. A. Tarzikian, and M. D. Frank-Kamenetskii, J. Biomol. Struct. Dyn. 8(1), 123 (1990).

    Google Scholar 

  11. E. B. Kruglova, N. A. Gladkovskaya, and V. Ya. Maleev, Biofizika 50(2), 253 (2005).

    Google Scholar 

  12. Yu. D. Nechipurenko, Mol. Biol. 18, 1066 (1984).

    Google Scholar 

  13. H.-J. Hinz and F. P. Schwarz, Pure Appl. Chem. 73(4), 745 (2001).

    Article  Google Scholar 

  14. J. D. McGhee, Biopolymers 15(7), 1345 (1976).

    Article  Google Scholar 

  15. L. A. Marky, K. S. Blumenfeld, and K. J. Breslauer, Nucl. Acids Res. 11(9), 2857 (1983).

    Article  Google Scholar 

  16. L. A. Marky, J. G. Snyder, and K. J. Breslauer, Nucl. Acids Res. 11(16), 5701 (1983).

    Article  Google Scholar 

  17. W. Muller and D. M. Crothers, Eur. J. Biochem. 54, 267 (1975).

    Article  Google Scholar 

  18. F. Hartley, C. Berges, and R. Olcock, Equilibria in Solutions (Mir, Moscow, 1983).

    Google Scholar 

  19. Ph. Hermann and E. Fredericq, Nucl. Acids Res. 4(8), 2939 (1977).

    Article  Google Scholar 

  20. P. McCall and V. A. Bloomfield, Biopolymers 15, 97 (1976).

    Article  Google Scholar 

  21. D. Esposito and P. Del Vicchio, J. Am. Chem. Soc. 119(11), 2606 (1997).

    Article  Google Scholar 

  22. R. R. Vadanyan, P. O. Vardevanyan, and G. A. Panosyan, Biofizika 29(3), 489 (1984).

    Google Scholar 

  23. L. A. Marky and K. J. Breslauer, Biopolymers 26, 1601 (1987).

    Article  Google Scholar 

  24. X. Shi and J. B. Chaires, Nucl. Acids Res. 34(2), e14 (2006).

    Article  Google Scholar 

  25. E. D. Horowitz and N. V. Hud, J. Am. Chem. Soc. 128, 1538 (2006).

    Article  Google Scholar 

  26. Y. Kubota, Y. Eguchi, K. Hashimoto, et al., Bull. Soc. Japan 49(9), 2424 (1976).

    Article  Google Scholar 

  27. D. O. Jordan and L. N. Sansom, Biopolymers 10(2), 399 (1971).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Radiophysics and Electronics, National Academy of Sciences of Ukraine, Kharkiv, 61085, Ukraine

    E. G. Bereznyak, N. A. Gladkovskaya, A. S. Khrebtova & E. V. Dukhopelnikov

  2. Institute of Problems of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine, Kharkiv, 61015, Ukraine

    A. V. Zinchenko

Authors
  1. E. G. Bereznyak
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. A. Gladkovskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. S. Khrebtova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. V. Dukhopelnikov
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. V. Zinchenko
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E. G. Bereznyak.

Additional information

Original Russian Text © E.G. Bereznyak, N.A. Gladkovskaya, A.S. Khrebtova, E.V. Dukhopelnikov, A.V. Zinchenko, 2009, published in Biofizika, 2009, Vol. 54, No. 5, pp. 805–812.

The article was translated by the authors.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bereznyak, E.G., Gladkovskaya, N.A., Khrebtova, A.S. et al. Peculiarities of DNA-proflavine binding under different concentration ratios. BIOPHYSICS 54, 574–580 (2009). https://doi.org/10.1134/S0006350909050030

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0006350909050030

Key words

Search

Navigation