Abstract
Osmotic adaptation in a mouse embryo blastomere has been studied by direct measurement of the cell volume using microtomography (laser scanning microscopy followed by quantitative 3D reconstruction). Embryo cells subjected to hypotonic shock first swelled but then returned to the initial size. At the beginning of osmotic stress, the swelling obeyed the van’t Hoff equation with a water permeability coefficient of 0.4 µm min−1 atm−1. The regulatory volume decrease was not abolished by Na+/K+-ATPase inhibition.
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References
M. Praissman, I. F. Miller, and J. M. Berkowitz, J. Membr. Biol. 11, 139 (1973).
M. Praissman, I. F. Miller, H. P. Gregor, and J. M. Berkowitz, J. Membr. Biol. 11, 153 (1973).
J. Loeb, J. Gen. Physiol. II, 387 (1920).
E. Grim and K. Sollner, J. Gen. Physiol. 40, 887 (1957).
B. Buckhold, R. B. Adams, and E. C. Gregg, Biochim. Biophys. Acta 102, 600 (1965).
H. M. Rosenberg, B. Buckhold Shank, and E. C. Gregg, J. Cell Physiol. 80, 23 (1972).
B. Buckhold Shank, H. M. Rosenberg, and C. Horowitz, J. Cell Physiol. 82, 257 (1973).
J. M. Baltz, J. D. Biggers, and C. Lechene, Development 118, 1353 (1993).
M. L. Day, M. H. Johnson, and D. I. Cook, EMBO J. 17, 1952 (1998).
A. J. Watson and G. M. Kidder, Dev. Biol. 126, 80 (1998).
L. J. Van Winkle and A. L. Campione, Dev. Biol. 146, 158 (1991).
J. D. Biggers, J. A. Lawitts, and C. P. Lechene, Mol. Reprod. Dev. 34, 380 (1993).
B. Fong, P. H. Watson, and A. J. Watson, BMC Dev. Biol. 7, 1 (2007).
A. G. Pogorelov and V. N. Pogorelova, J. Microsc. 232, 36 (2008).
M. Munk, Mammalian Development Biology (Mir, Moscow, 1990).
A. G. Pogorelov, B. L. Allachverdov, I. V. Burovina, et al., J. Microsc. 162, 255 (1991).
A. G. Pogorelov, V. N. Pogorelova, N. V. Repin, and I. A. Mizin, Scanning Microscopy Suppl. 8, 101 (1994).
A. G. Pogorelov and D. V. Goldshtein, Klet. Tekhnol. Biol. Med., no. 2, 84 (2006).
A. G. Pogorelov, I. I. Katkov, E. I. Smolyaninova, and D. V. Goldshtein, CryoLetters 27, 87 (2006).
A. G. Pogorelov, I. I. Katkov, and V. N. Pogorelova, CryoLetters 28, 403 (2007).
A. M. Glauert and P. R. Lewis, Biological Specimen Preparation for Transmission Electron Microscopy (Princeton Univ. Press, Princeton, 1998).
M. A. Pogorelova, V. A. Yashin, A. G. Pogorelov, and V. A. Golichenkov, Dokl. RAN 418, 712 (2008).
D. Y. Gao, C. T. Benson, C. Liu, et al., Biophys. J. 71, 443 (1996).
Y. Agca, C. Liu, J. J. McGrath, et al., Cryobiology 36, 287 (1998).
C. Slack and A. E. Warner, J. Physiol. 232, 313 (1973).
K. L. Cahalin and J. E. Hall, Biophys. J. 76, 183 (1999).
M. Yasui, T. H. Kwon, M. A. Knepper, et al., Proc. Natl. Acad. Sci. USA 96, 5808 (1999).
I. Yazaki, E. Tosti, and B. Dale, Development 121, 1827 (1995).
D. Kondratev and M. F. Gallitelli, Cell Calcium, 34, 193 (2003).
O. Y. Samoilov, Structure of Aqueous Electrolyte Solutions and Hydration of Ions (Consultants Bureau, New York, 1965).
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Original Russian Text © A.G. Pogorelov, V.N. Pogorelova, 2009, published in Biofizika, 2009, Vol. 54, No. 3, pp. 482–487.
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Pogorelov, A.G., Pogorelova, V.N. Dynamics of cell volume in early mouse embryos subjected to hypotonic shock. BIOPHYSICS 54, 336–339 (2009). https://doi.org/10.1134/S0006350909030130
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DOI: https://doi.org/10.1134/S0006350909030130