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Experimental verification of the model of complement-dependent immune lysis of liposomes

  • Cell Biophysics
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Abstract

The quantitative dependences of the complement-dependent immune lysis of a monodisperse suspension of 200-nm liposomes sensitized by a monovalent hapten (2,4-DNP-ɛ-caproyl-DPPE) or a polyvalent antigen (LPS from Francisella tularensis) on the initial concentration of specific antibodies (IgG) to the hapten and antigen have been investigated. The quantity of antibody-binding sites on the liposome surface was evaluated. The difference between the complement-dependent lysis of poly- and monodisperse suspensions of liposomes was shown. The experimental results are well described by the direct binding model.

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Abbreviations

Ag:

antigen(s)

Ab:

antibody(ies)

CDL:

complement-dependent immune lysis

DNPC-DPPE:

2,4-dinitrophenol-ɛ-caproyl-dipalmitoylphosphatidylethanolamine

LPS:

lipopolysaccharide

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Authors and Affiliations

  1. State Research Institute of Biological Engineering, Moscow, 125424, Russia

    S. P. Yarkov & S. N. Skopinskaya

Authors
  1. S. P. Yarkov
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  2. S. N. Skopinskaya
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Correspondence to S. P. Yarkov.

Additional information

Original Russian Text © S.P. Yarkov, S.N. Skopinskaya, 2009, published in Biofizika, 2009, Vol. 54, No. 3, pp. 459–463.

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Yarkov, S.P., Skopinskaya, S.N. Experimental verification of the model of complement-dependent immune lysis of liposomes. BIOPHYSICS 54, 320–322 (2009). https://doi.org/10.1134/S0006350909030105

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  • DOI: https://doi.org/10.1134/S0006350909030105

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