Skip to main content
SpringerLink
Log in

The contribution of protein kinase C and rho-kinase to the regulation of receptor-dependent contraction of arteries decreases with age independently of sympathetic innervation

  • Complex Systems Biophysics
  • Published:
Biophysics Aims and scope Submit manuscript

Abstract

The age-related dynamics of the activity of signaling pathways coupled with α1-adrenoreceptors and their dependence on sympathetic innervation of arterial smooth muscle has been studied. For this purpose, the effects of protein kinase C inhibitor (GF 109203X, 10−6 M and Rho-kinase inhibitor (Y27632, 10−5 M) on isometric contraction of the rat saphenous artery in response to the α1-adrenoreceptor agonist methoxamine were determined. The rats in the age of two weeks (with partially developed sympathetic innervation) had the lower vascular sensitivity to methoxamine than adult rats, but the effects of both inhibitors were more prominent. The denervation induced by excision of sympathetic ganglia in adult rats increased the arterial sensitivity to methoxamine but the sensitivity to inhibitors was unchanged. Thus, the postnatal development of the arterial smooth muscle is accompanied by diminution of the role of protein kinase C and Rho-kinase in the regulation of contraction, but these changes do not correlate with the changes in arterial sensitivity to α1-adrenergic stimulation and development of sympathetic innervation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

α-AR:

α-adrenoreceptors

[Ca2+]i :

intracellular concentration of free Ca2+

PKC:

protein kinase C

RhoK:

Rho kinase (kinase activated by the Rho protein)

MLC:

myosin light chains

MAPK:

mitogen-activated protein kinases

References

  1. G. K. Owens, M. S. Kumar, and B. R. Wamhoff, Physiol. Rev. 84, 767 (2004).

    Article  Google Scholar 

  2. R. D. Bevan, Hypertension 6(Suppl. III), III–1 (1984).

    Google Scholar 

  3. D. H. Damon, Am. J. Physiol. 288, H2785 (2005).

    ADS  Google Scholar 

  4. M. E. Todd, J. Anat. 131, 121 (1980).

    Google Scholar 

  5. D. R. Varma and X. F. Deng, Can. J. Physiol. Pharmacol. 78, 267 (2000).

    Article  Google Scholar 

  6. S. M. Yu, S. Y. Tsai, J. H. Guh, et al., Circulation 94, 547 (1996).

    Google Scholar 

  7. J. K. Phillips, M. Vidovic, and C. E. Hill, Mech. Ageing Dev. 92, 235 (1996).

    Article  Google Scholar 

  8. J. K. Phillips and C. E. Hill, Int. J. Dev. Neurosci. 17, 377 (1999).

    Article  Google Scholar 

  9. F. R. Stassen, R. G. Maas, P. M. Schiffers, et al., J. Pharmacol. Exp. Ther. 284, 399 (1998).

    Google Scholar 

  10. T. M. Seasholtz, M. Majumdar, and J. H. Brown, Mol. Pharmacol. 55, 949 (1999).

    Google Scholar 

  11. A. V. Vorotnikov, M. A. Krymskii, and V. P. Shirinskii, Biokhimiya 67(12), 1587 (2002).

    Google Scholar 

  12. A. P. Somlyo and A. V. Somlyo, Physiol. Rev. 83, 1325 (2003).

    Google Scholar 

  13. N. B. Standen and J. M. Quayle, Acta Physiol. Scand. 164, 549 (1998).

    Article  Google Scholar 

  14. K. D. Luykenaar, S. E. Brett, B. N. Wu, et al., Am. J. Physiol. 286, H1088 (2004).

    Google Scholar 

  15. B. Y. Su, K. M. Reber, C. A. Nankervis, and P. T. Nowicki, Am. J. Physiol. 284, G445 (2003).

    Google Scholar 

  16. J. Belik, E. Kerc, and M. D. Pato, Am. J. Physiol. 290, L509 (2005).

    Google Scholar 

  17. M. Ekman, K. Fagher, M. Wede, et al., J. Gen. Physiol. 125, 187 (2005).

    Article  Google Scholar 

  18. R. J. Sandoval, E. R. Injeti, W. T. Gerthoffer, and W. J. Pearce, Am. J. Physiol. 293, H2183 (2007).

    Google Scholar 

  19. M. C. Payne, H. Y. Zhang, T. Prosdocimo, et al., J. Mol. Cell Cardiol. 40, 274 (2006).

    Article  Google Scholar 

  20. I. Mueed, P. Bains, L. Zhang, and K. M. MacLeod, Can. J. Physiol. Pharmacol. 82, 895 (2004).

    Article  Google Scholar 

  21. S. T. Abraham, M. Robinson, and P. J. Rice, Pharmacology 67, 32 (2003).

    Article  Google Scholar 

  22. K. Kacem and R. Sercombe, Auton. Neurosci. 124, 38 (2006).

    Article  Google Scholar 

  23. O. S. Tarasova, V. A. Puzdrova, V. U. Kalenchuk, and V. B. Koshelev, Biofizika 51(5), 912 (2006).

    Google Scholar 

  24. J. Q. Griffith, E. J. Farris, and J. B. Lippincott, in The Rat in Laboratory Investigation (Philadelphia-Montreal-London, 1942).

  25. M. J. Mulvany and W. Halpern, Circ. Res. 41, 19 (1977).

    Google Scholar 

  26. M. Gollasch, H. Haase, C. Ried, et al., FASEB J. 12, 593 (1998).

    Google Scholar 

  27. J. F. Quignard, E. Grazzini, G. Guillon, et al., Pflugers Arch. 431, 791 (1996).

    Article  Google Scholar 

  28. S. Dakshinamurti, L. Mellow, and N. L. Stephens, Pediatric Pulmonology 40, 398 (2005).

    Article  Google Scholar 

  29. W. J. Pearce, J. M. Williams, M. M. Chang, and W. T. Gerthoffer, Arch. Physiol. Biochem. 111, 36 (2003).

    Article  Google Scholar 

  30. W. W. Fleming, J. Pharmacol. Exp. Ther. 291, 925 (1999).

    Google Scholar 

  31. K. Ramos, W. T. Gerthoffer, and D. P. Westfall, J. Pharmacol. Exp. Ther. 236, 80 (1985).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biological Department, Moscow State University, Moscow, 119991, Russia

    S. V. Mochalov & D. K. Gainullina

  2. Department of Fundamental Medicine, Moscow State University, Lomonosovskii pr. 31, korp. 5, Moscow, 119192, Russia

    V. U. Kalenchuk & A. V. Vorotnikov

  3. Institute of Biomedical Problems, Russian Academy of Sciences, State Research Center of Russian Federation, Khoroshevskoe sh. 76A, Moscow, 123007, Russia

    O. S. Tarasova

Authors
  1. S. V. Mochalov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. U. Kalenchuk
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. K. Gainullina
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. V. Vorotnikov
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. O. S. Tarasova
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to O. S. Tarasova.

Additional information

Original Russian Text © S.V. Mochalov, V.U. Kalenchuk, D.K. Gainullina, A.V. Vorotnikov, O.S. Tarasova, 2008, published in Biofizika, 2008, Vol. 53, No. 6, pp. 1102–1108.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mochalov, S.V., Kalenchuk, V.U., Gainullina, D.K. et al. The contribution of protein kinase C and rho-kinase to the regulation of receptor-dependent contraction of arteries decreases with age independently of sympathetic innervation. BIOPHYSICS 53, 626–631 (2008). https://doi.org/10.1134/S0006350908060298

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0006350908060298

Key words

Search

Navigation