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Optical trap as a tool for studying motor proteins

  • Molecular Biophysics
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Abstract

The review briefs the optical trap method, a modern experimental tool based on the recently discovered ability of light to trap and hold micron and submicron particles in a focused beam. The physical principle underlying the optical trap and the opportunities that it provides for studying the molecular nature of biological motility are considered. Several studies into the physical characteristics and functions of single motor protein molecules performed using the optical trap and recording nanometer displacements and piconewton forces are analyzed.

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Abbreviations

NEM:

N-ethylmaleimide

HMM:

heavy meromyosin

AOM:

acoustooptical modulator

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Authors and Affiliations

  1. Institute of Immunology and Physiology, Ural Division, Russian Academy of Sciences, ul. Pervomaiskaya 91, Yekaterinburg, 620041, Russia

    S. R. Nabiev, D. A. Ovsyannikov, B. Y. Bershitsky & S. Y. Bershitsky

Authors
  1. S. R. Nabiev
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  2. D. A. Ovsyannikov
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  3. B. Y. Bershitsky
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  4. S. Y. Bershitsky
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Correspondence to S. R. Nabiev.

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Original Russian Text © S.R. Nabiev, D.A. Ovsyannikov, B.Y. Bershitsky, S.Y. Bershitsky, 2008, published in Biofizika, 2008, Vol. 53, No. 6, pp. 929–935.

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Nabiev, S.R., Ovsyannikov, D.A., Bershitsky, B.Y. et al. Optical trap as a tool for studying motor proteins. BIOPHYSICS 53, 488–493 (2008). https://doi.org/10.1134/S0006350908060031

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  • DOI: https://doi.org/10.1134/S0006350908060031

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