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Aggregation of crystallins induced by pulsed laser UV light (308 nm)

  • Molecular Biophysics
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Abstract

Here we compile and analyze the data on photoaggregation of a model protein carboanhydrase and the main eye lens proteins α-, β-, γ-crystallins under the action of pulsed UV irradiation from a Xe-Cl laser (308 nm) with broad variation of pulse energy density and repetition rate. The aggregation efficacy proves to be a nonlinear function of these parameters and protein concentration. A theoretical model is proposed that qualitatively explains the experimental data. It is shown that N-arm-truncated βA3-crystallin is more prone to UV-induced aggregation than the full-sized protein; such defects caused by mutation or aging may aggravate the development of lenticular opacity. Analyzed is the effect of some low-molecular compounds on the aggregation of β-crystallin and its mixture with α-crystallin. A combination of short peptides prepared on this basis markedly impedes crystallin aggregation and retards the development of UV-induced cataract in rats.

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Authors and Affiliations

  1. Institute of Applied Physics, Russian Academy of Sciences, Nizhni Novgorod, 603950, Russia

    L. V. Soustov, E. V. Chelnokov, N. V. Sapogova & N. M. Bityurin

  2. Blokhina Institute of Epidemiology and Microbiology, Nizhni Novgorod, 603950, Russia

    V. V. Nemov

  3. National Eye Institute, NIH, Bethesda, MD, 20892-2510, USA

    Yu. V. Sergeev

  4. Emmanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, 119334, Russia

    M. A. Ostrovsky

Authors
  1. L. V. Soustov
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  2. E. V. Chelnokov
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  3. N. V. Sapogova
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  4. N. M. Bityurin
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  5. V. V. Nemov
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  6. Yu. V. Sergeev
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  7. M. A. Ostrovsky
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Original Russian Text © L.V. Soustov, E.V. Chelnokov, N.V. Sapogova, N.M. Bityurin, V.V. Nemov, Yu.V. Sergeev, M.A. Ostrovsky, 2008, published in Biofizika, 2008, Vol. 53, No. 4, pp. 582–597.

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Soustov, L.V., Chelnokov, E.V., Sapogova, N.V. et al. Aggregation of crystallins induced by pulsed laser UV light (308 nm). BIOPHYSICS 53, 273–282 (2008). https://doi.org/10.1134/S0006350908040064

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  • DOI: https://doi.org/10.1134/S0006350908040064

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