Abstract
A study was made of the inclusion of fluorescent probe H-510 based on 3,3′-dialkyloxacarbocyanine bromide (where alkyl is ethyl, nonyl, or octadecyl) into cells of different types. Alkyl chain length (C2, C9, or C18) was found to largely determine the accumulation dynamics and mechanism. Similar spectral characteristics for all probe types in bone marrow cells were found by microfluorimetry, suggesting insertion of dye molecules irrespective of their lipophilicity into micelle-like structures formed probably by cell phospholipids. Spectroscopy data indicate interaction of 3,3′-diethyloxacarbocyanine bromide (H-510/C2) and 3,3-dinonyloxacarbocyanine bromide (H-510/C9) dyes in hepatocytes with a less polar microenvironment (nonpolar and low-polar lipids that constitute a significant part of the total content of cell lipids). The fluoresccence maximum of long-chain dye H-510/C18 in hepatocytes is shifted to the short-wavelength region and strictly coincides with the fluorescence maximum of the probe in an albumin solution. It is not excluded that inclusion of the probe into cells occurs via endocytosis upon its binding to surface proteins.
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Original Russian Text © Yu.V. Malyukin, I.A. Borovoi, N.S. Kavok, A.V. Gerashchenko, N.L. Pogrebnyak, S.L. Efimova, A.N. Lebedenko, 2007, published in Biofizika, 2007, Vol. 52, No. 4, pp. 667–673.
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Malyukin, Y.V., Borovoi, I.A., Kavok, N.S. et al. Accumulation of oxacarbocyanine dyes with different alkyl chain length in bone marrow cells and hepatocytes. BIOPHYSICS 52, 406–411 (2007). https://doi.org/10.1134/S0006350907040082
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DOI: https://doi.org/10.1134/S0006350907040082