Abstract
The fluorescence of β-carotene/chlorophyll a mixtures in complex with bovine serum albumin in water solution was found to exceed that of one-pigment complexes, being maximal at initial Car/Chl molar ratios of 8–4, whereas the amount of pigment adsorbed on BSA was maximal for an equimolar mixture. The fluorescence spectra of Car-BSA and Chl-BSA complexes largely overlapped (maxima at 684 and 690 nm, respectively).
Similar content being viewed by others
Abbreviations
- Car:
-
β-carotene
- Chl:
-
chlorophyll a
References
V. D. Semichaevskii, Biofizika 17, 539 (1972).
E. I. Zen’kevich, G. A. Kochubeev, and K. I. Salokhiddinov, Zh. Prikl. Spektrosk. 29, 639 (1978).
H. Shibata, H. Ochiai, T. Kawashima, et al., Biochim. Biophys. Acta 852(2–3), 175 (1986).
A. White, F. Handler, E. Smith, et al., Basic Biochemistry (Mir, Moscow, 1981), vol. 3.
A. K. Chibisov, T. D. Slavnova, and H. Gorner, J. Photochem. Photobiol. B: Biol. 72, 11 (2003).
F. Zsila, M. Simonyi, S. F. Lockwood, Bioorg. Med. Chem. Lett. 13, 4093 (2003).
J. Sowell, K. A. Agrew-Heard, J. C. Mason, et al., J. Chromatogr. B 755, 91 (2001).
I. E. Borissevitch, T. T. Tominaga, and C. C. Schmitt, J. Photochem. Photobiol. A: Chem. 114, 201 (1998).
M. D. Harvey, V. Bablekis, P. R. Banks, and C. D. Skinner, J. Chromatogr. B. 754, 345 (2001).
I. E. Borissevitch, T. T. Tominaga, H. Isamoto, and M. Tabak, J. Luminescence. 69, 65 (1996).
I. E. Borissevitch, T. T. Tominaga, H. Isamoto, and M. Tabak, Anal. Chim. Acta 343, 281 (1997).
N. G. Karapetyan and V. N. Madakyan, Bioorg. Khim. 30, 195 (2004).
I. A. Nagovitsyn and G. K. Chudinova, Dokl. RAN 382, 267 (2002).
G. K. Chudinova, I. A. Nagovitsyn, R. E. Karpov, and V. V. Savranskii, Kvant. Elektron. 33, 765.
I. A. Nagovitsyn, G. K. Chudinova, and G. G. Komissarov, Dokl. RAN 382, 115 (2002).
I. A. Nagovitsyn, G. K. Chudinova, V. V. Savranskii, and G. G. Komissarov, Biofizika 49, 646 (2004).
I. A. Nagovitsyn, G. K. Chudinova, V. V. Savranskii, and G. G. Komissarov, Kr. Soobshch. Fiz. FIAN 186(11), 19 (2003).
L. Langmuir and V. J. Schaefer, J. Am. Chem. Soc. 58(1), 284 (1936).
I. A. Nagovitsyn, G. K. Chudinova, V. D. Rumyantseva, V. A. Ilatovskii, and G. G. Komissarov, Biofizika 47, 625 (2002).
V. D. Semichaevskii, Mol. Biol. 9, 351 (1975).
F. F. Litvin, B. A. Gulyaev, and V. A. Sineshchekov, Dokl. AN SSSR 162, 1184 (1965)
Chemical Encyclopedia, Ed. by I. L. Knunyants (Sov. Entsiklopediya, Moscow, 1990), vol. 2 [in Russian].
R. Bensasson, E. Land, and T. Truscot, Flash Photolysis and Pulse Radiolysis: Application in Biochemistry and Medical Chemistry (Mir, Moscow, 1987).
E. Stern and K. Timmons, Electron Absorption Spectroscopy in Organic Chemistry (Mir, Moscow, 1974).
E. Rabinovich, Photosynthesis (Inostr. Lit., Moscow, 1953), vol. 2.
R. M. C. Dawson, D. C. Elliott, W. H. Elliott, and K. M. Jones, Data for Biochemical Research, 3rd Edn. (Clarendon Press, Oxford, UK, 1986).
V. A. Sineshchekov, F. F. Litvin, and M. Das, Photochem. Photobiol. 15, 187 (1972).
Yu. E. Giller, Biofizika 13, 1006 (1968).
Yu. E. Giller, G. V. Krasichekova, and D. I. Sapozhnikov, Biofizika 15, 38 (1970).
T. Larkum, Trends Plant Sci. 1(8), 247 (1996).
A. Damjanović, T. Ritz, and K. Schulten, Biophys. J. 79, 1695 (2000).
Author information
Authors and Affiliations
Additional information
Original Russian Text © I.A. Nagovitsyn, G.K. Chudinova, V.V. Savranskii, G.G. Komissarov, 2007, published in Biofizika, 2007, Vol. 52, No. 4, pp. 643–649.
Rights and permissions
About this article
Cite this article
Nagovitsyn, I.A., Chudinova, G.K., Savranskii, V.V. et al. Optical properties of chlorophyll a and β-Carotene mixtures adsorbed on bovine serum albumin. BIOPHYSICS 52, 389–392 (2007). https://doi.org/10.1134/S0006350907040057
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1134/S0006350907040057