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Corroboration of the functional role of the additional zinc binding site in the G245C mutant form of the p53 protein

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Abstract

Our earlier suggestion that the G245C mutation in p53 generates an additional zinc binding site, overlapping with the normal zinc binding site, has been supported by molecular modeling. The energy of interaction between a zinc ion and the new site in the G245 mutant is comparable to that for the normal site in wild-type p53. The presence of the additional site in the mutant can distort its conformation when it interacts with DNA. Effects of other mutations on the energy of zinc binding to the normal site have been calculated.

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References

  1. W. S. El-Deiry, S. E. Kern, J. A. S. L. Pietenpol, et al., Nat. Genet. 1, 45 (1992).

    Article  Google Scholar 

  2. A. J. Giaccia and M. B. Kastan, Genes Dev. 12, 2973 (1998).

    Article  Google Scholar 

  3. S. Jin and A. J. Levine, J. Cell Sci. 114, 4139 (2001).

    Google Scholar 

  4. A. S. Joerger, M. D. Allen, and A. R. Fersht, J. Biol. Chem. 279, 1291 (2004).

    Article  Google Scholar 

  5. P. Hainaut and M. Hollstein, Adv. Cancer Res. 77, 81 (2000).

    Article  Google Scholar 

  6. A. C. R. Martin, A. M. Facchiano, A. L. Cuff, et al., Hum. Mutat. 19, 149 (2002).

    Article  Google Scholar 

  7. S. E. Kern, J. A. Pietenpol, S. Thiagalingam, et al., Science 256, 827 (1992).

    Article  ADS  Google Scholar 

  8. K. B. Wong, B. S. DeDecker, S. M. Freund, et al., Proc. Natl. Acad. Sci. USA 96, 8438 (1999).

    Article  ADS  Google Scholar 

  9. A. N. Bullock, J. Henckel, B. S. DeDecker, et al., Proc. Natl. Acad. Sci. USA 94, 14338 (1997).

    Article  ADS  Google Scholar 

  10. A. Friedler, D. B. Veprintsev, L. O. Hansson, and A. R. Fersht, J. Biol. Chem. 278, 24108 (2003).

    Article  Google Scholar 

  11. J. S. Butler and S. N. Loh, Biochemistry 42, 2396 (2003).

    Article  Google Scholar 

  12. V. A. Ivanisenko, S. S. Pintus, D. A. Grigorovich, and N. A. Kolchanov, Nucleic Acids Res. 33, D183 (2005).

    Article  Google Scholar 

  13. A. A. Canutescu, A. A. Shelenkov, and R. L. Dunbrack, Jr., Protein Science 12, 2001 (2003).

    Article  Google Scholar 

  14. V. A. Ivanisenko, S. S. Pintus, D. A. Grigorovich, and N. A. Kolchanov, Nucleic Acids Res. 32, W549 (2004).

    Article  Google Scholar 

  15. http://amber.scripps.edu/dbase.html.

  16. M. J. S. Dewar and W. Thiel, J. Am. Chem. Soc. 99, 4899 (1977).

    Article  Google Scholar 

  17. Y. Yueting and X. Chengxian, Eur. J. Operat. Res. 180(11), 48 (2006).

    MathSciNet  Google Scholar 

  18. J. W. Ponder and D. A. Case, Adv. Prot. Chem. 66, 27 (2003).

    Google Scholar 

  19. D. Frenkel, in Molecular Dynamics Simulation of Statistical Mechanical Systems, XCVII (Soc. Italiana di Fisica, Bologna, Italy, 1986).

    Google Scholar 

  20. D. Malkin, F. P. Li, L. C. Strong, J. F. Fraumeni, Jr., et al., Science 250, 1233 (1990).

    Article  ADS  Google Scholar 

  21. T. Frebourg, J. Kassel, K. T. Lam, et al., Proc. Natl. Acad. Sci. USA 89, 6413 (1992).

    Article  ADS  Google Scholar 

  22. I. B. Bersuker and A. V. Ablov, Chemical Bond in Coordinated Compounds (Kishinev, 1962) [in Russian].

  23. J. Gao, Rev. Comp. Chem. 7, 119 (1996).

    Article  Google Scholar 

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, pr. akademika Lavrent’eva 10, Novosibirsk, 630090, Russia

    E. S. Fomin & V. A. Ivanisenko

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  1. E. S. Fomin
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  2. V. A. Ivanisenko
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Fomin, E.S., Ivanisenko, V.A. Corroboration of the functional role of the additional zinc binding site in the G245C mutant form of the p53 protein. BIOPHYSICS 51 (Suppl 1), 35–38 (2006). https://doi.org/10.1134/S0006350906070074

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