Abstract
The structure of mitochondrial cristae has been studied for the first time by the method of small-angle neutron scattering. Experiments were performed on intact (functioning) mitochondria from rat liver. Mitochondrial cristae are usually considered to be folds of the inner membrane with arbitrary variable intermembrane distances. Under conditions of low-amplitude swelling, mitochondrial cristae transformed into double-membrane structures with a distance of 190 Å between the central planes of the membranes. The formation of double-membrane structures and their structural parameters did not depend on the method for inducing swelling which was accomplished either by placing the mitochondria into a hypotonic medium or through the opening of nonspecific pores.
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F. Lang, E. Gulbins, I. Szabo, and A. Lepple-Wienhues, J. Mol. Recognit. 17(5), 473 (2004).
A. P. Halestrap, P. T. Quinlan, D. E. Whipp, and A. E. Armston, Biochem. J. 236(3), 779 (1986).
P. T. Quinlan, A. P. Thomas, A. E. Armston, and A. P. Halestrap, Biochem. J. 214(2), 395 (1983).
P. Korge, H. M. Honda, and J. N. Weiss, Am. J. Physiol. Heart Circ. Physiol. 289(1), 66 (2005).
S. N. Orlov, N. I. Pokudin, Y. V. Kotelevtsev, and P. V. Gulak, J. Membr. Biol. 107(2), 105 (1989).
D. E. Gulham, J. Henderson, R. A. Crane, and J. M. Wood, Biochemistry 42(2), 410 (2003).
I. P. Krasinskaya, V. N. Marshanscky, S. F. Dragunova, and L. S. Yaguzhinsky, Biokhimiya 49(1), 87 (1984).
I. P. Krasinskaya, V. N. Marshanscky, S. F. Dragunova, and L. S. Yaguzhinsky, FEBS Lett. 167(1), 176 (1984).
Yu. A. Vladimirov and G. E. Dobretsov, Fluorescent Probes in Studies on Biological Membranes (Nauka, Moscow, 1980) [in Russian].
I. P. Krasinskaya, I. S. Litvinov, S. D. Zakharov, L. E. Bakeeva, and L. S. Yaguzhinsky, Biokhimiya 54(9), 1550 (1989).
K. D. Garlid, Biochem. Biophys. Res. Commun. 83(4), 1450 (1978).
G. P. Brierly, M. S. Jurkowitz, T. Farooqui, and D.W. Jung, J. Biol. Chem. 259(23) 14672 (1984).
J. P. Williams, J. Theor. Biol. 219, 389 (2002).
C. A. Mannella and D. F. Parsons, Biochim. Biophys. Acta 460(2), 375 (1977).
C. A. Mannella and D. F. Parsons, Biochim. Biophys. Acta 470(2), 242 (1977).
D. Jonson and H. Lardy, Methods Enzymol. 10, 90 (1967).
A. I. Kuklin, A. Kh. Islamov, and V. I. Gordeliy, Neutron News 16(3), 16 (2005).
A. G. Solov’ev, T. M. Solov’eva, A. V. Stadnik, et al., Preprint R10-2003-86, OIYaI (Joint Institute of Nuclear Research, Dubna, 2003).
P. W. Schmidt, Modern Aspects of Small-Angle Scattering, Ed. by H. Brumberger (Kluwer, Dordrecht, 1995), pp. 1–56.
G. Porod, Kolloid. Zh. 124, 83 (1951).
D. I. Svergun and L. A. Feigin, X-Ray and Neutron Small-Angle Scattering (Nauka, Moscow, 1986) [in Russian].
C. R. Hackenbrock, Biochemistry 61, 598 (1968).
M. Crompton, S. Virji, V. Doyle, N. Johnson, and J. M. Ward, Biochem. Soc. Symp. 66, 167 (1999).
A. E. Lyubarev and V. I. Kurganov, Biosystems 22(2), 91 (1989).
J. P. Williams, J. Theor. Biol. 1, 1 (1961).
I. M. Solodovnikova, V. I. Yurkov, A. A. Ton’shin, and L. S. Yaguzhinsky, Biofizika 49(1), 47 (2004) [Biophysics 49 (1), 42 (2004)].
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Original Russian Text © T.N. Murugova, V.I. Gordeliy, A.I. Kuklin, Yu.S. Kovalev, V.I. Yurkov, A. Nurenberg, A.Kh. Islamov, L.S. Yaguzhinskii, 2006, published in Biofizika, 2006, Vol. 51, No. 6, pp. 1001–1007.
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Murugova, T.N., Gordeliy, V.I., Kuklin, A.I. et al. Detection of new double-membrane structures in native mitochondria by the method of small-angle neutron scattering. BIOPHYSICS 51, 882–886 (2006). https://doi.org/10.1134/S0006350906060054
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DOI: https://doi.org/10.1134/S0006350906060054