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Diversity and Similarity of Termination and Ribosome Rescue in Bacterial, Mitochondrial, and Cytoplasmic Translation

Biochemistry (Moscow) volume 86, pages 1107–1121 (2021)Cite this article

Abstract

When a ribosome encounters the stop codon of an mRNA, it terminates translation, releases the newly made protein, and is recycled to initiate translation on a new mRNA. Termination is a highly dynamic process in which release factors (RF1 and RF2 in bacteria; eRF1•eRF3•GTP in eukaryotes) coordinate peptide release with large-scale molecular rearrangements of the ribosome. Ribosomes stalled on aberrant mRNAs are rescued and recycled by diverse bacterial, mitochondrial, or cytoplasmic quality control mechanisms. These are catalyzed by rescue factors with peptidyl-tRNA hydrolase activity (bacterial ArfA•RF2 and ArfB, mitochondrial ICT1 and mtRF-R, and cytoplasmic Vms1), that are distinct from each other and from release factors. Nevertheless, recent structural studies demonstrate a remarkable similarity between translation termination and ribosome rescue mechanisms. This review describes how these pathways rely on inherent ribosome dynamics, emphasizing the active role of the ribosome in all translation steps.

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Acknowledgments

I thank Anna Loveland and Darryl Conte Jr. for comments on the manuscript.

Funding

This work was financially supported by the Cystic Fibrosis Foundation (CFF; grant 670773) and by the National Institutes of Health (grant R35 GM127094).

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Authors and Affiliations

  1. RNA Therapeutics Institute, Department of Biochemistry and Molecular Pharmacology, UMass Medical School, Worcester, MA, USA

    Andrei A. Korostelev

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  1. Andrei A. Korostelev
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Correspondence to Andrei A. Korostelev.

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The author declares no conflict of interest in financial or any other sphere. This article does not contain description of studies with humans participants or animals performed by the author.

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Korostelev, A.A. Diversity and Similarity of Termination and Ribosome Rescue in Bacterial, Mitochondrial, and Cytoplasmic Translation. Biochemistry Moscow 86, 1107–1121 (2021). https://doi.org/10.1134/S0006297921090066

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  • DOI: https://doi.org/10.1134/S0006297921090066

Keywords

  • translation
  • termination
  • ribosome
  • rescue