Abstract
Binding of dinitrosyl iron complex (DNIC) to albumin was studied using time-resolved fluorescence (TRF) and electron spin resonance (ESR) spectroscopy. It was found that the fluorescence lifetime of bovine serum albumin (BSA) and human serum albumin (HSA) decreases with binding and depends on DNIC concentration. The observed biexponential pattern of the BSA tryptophan (Trp) fluorescence decay is explained by the presence of two tryptophan residues in the protein molecule. We believe that DNIC forms stable complexes with the cysteine (Cys34) residue in the domain I of albumin. It was shown that the lifetime of albumin tryptophan fluorescence decreased during co-incubation of BSA with DNICs and glutathione. Effects of DNIC on the binding of specific spin-labeled fatty acids with albumin in human blood plasma were studied in vitro. The presence of DNIC in blood plasma does not change conformation of albumin domains II and III. We suggest that the most possible interaction between DNICs and albumin is the formation of a complex; and nitrosylation of the cysteine residue in the albumin domain I occurs without the changes in albumin conformation.
Abbreviations
- BSA:
-
bovine serum albumin
- DNIC:
-
dinitrosyl iron complex
- ESR:
-
electron spin resonance
- GS-NO:
-
S-nitroso glutathione
- HAS:
-
human serum albumin
- TRF:
-
time-resolved fluorescence
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Acknowledgments
The authors are grateful to Dr. Margarita Rodionova for her invaluable help in preparation of the manuscript.
Funding
This study was financially supported by the Russian Science Foundation (grant no. 19-79-30062, for M.G.V. and Y.A.I.) as well as by the Interdisciplinary Scientific and Educational School of Moscow University “Molecular Technologies of Living Systems and Synthetic Biology”.
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The authors declare no conflicts of interest in financial or any other sphere. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
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Allakhverdiev, E.S., Maksimov, G.V., Rodnenkov, O.V. et al. Effect of Dinitrosyl Iron Complex on Albumin Conformation. Biochemistry Moscow 86, 533–539 (2021). https://doi.org/10.1134/S0006297921050023
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DOI: https://doi.org/10.1134/S0006297921050023