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Biochemistry (Moscow)

, Volume 83, Issue 6, pp 766–777 | Cite as

Identification and Characterization of MicroRNAs in Skin of Chinese Giant Salamander (Andrias davidianus) by the Deep Sequencing Approach

  • Yong Huang
  • Wang Bao Gong
Article
  • 21 Downloads

Abstract

MicroRNAs (miRNA) play a pivotal role in regulating a broad range of biological processes, acting by cleaving mRNAs or by translational repression. However, the miRNAs from skin of Andrias davidianus have not been reported. In this study, a small-RNA cDNA library was constructed and sequenced from skin of A. davidianus. A total of 513 conserved miRNAs belonging to 174 families were identified. The remaining 108 miRNAs we identified were novel and likely to be skin tissue-specific but were expressed at low levels. The presence of randomly selected 15 miRNAs identified and their expression in eight different tissues from A. davidianus were validated by stem-loop qRT-PCR. For better understanding the functions of miRNAs, 129,791 predicated target genes were analyzed by GO and their pathways illustrated by KEGG pathway analyses. The results show that these identified miRNAs from A. davidianus skin are involved in a broad range of physiological functions including metabolism, growth, development, and immune responses. This study exhaustively identifies miRNAs and their target genes, which will ultimately pave the way for understanding their role in skin of A. davidianus and other amphibians. Further studies are necessary to better understand miRNA-mediated gene regulation.

Keywords

Andrias davidianus microRNA skin deep sequencing target gene 

Abbreviations

GO analysis

gene ontology enrichment analysis

KEGG analysis

Kyoto Encyclopedia of Genes and Genomes pathway analysis

miRNA

microRNA

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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  1. 1.College of Animal Science and TechnologyHenan University of Science and TechnologyLuoyangChina
  2. 2.Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Pearl River Fisheries Research InstituteChinese Academy of Fishery SciencesGuangzhouChina

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