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Biochemistry (Moscow)

, Volume 83, Issue 6, pp 727–737 | Cite as

Localization of Galectins within Glycocalyx

  • E. M. Rapoport
  • V. K. Matveeva
  • O. A. Vokhmyanina
  • I. M. Belyanchikov
  • H.-J. Gabius
  • N. V. Bovin
Article
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Abstract

Galectins are involved in various biological processes, e.g. cell–cell and cell–matrix adhesion and the transmission of cellular signals. Despite the diversity of functions, little is known about the nature of their physiological cognate ligands on the cell surface and the localization of galectins in the glycocalyx, although this information is important for understanding the functional activity of galectins. In this work, localization of endogenous and exogenously loaded galectins in the glycocalyx was studied. The following main conclusions are drawn: 1) galectins are not evenly distributed within the glycocalyx, they are accumulated in patches. Patching is not the result of a cross-linking of cellular glycans by galectins. Instead, patch-wise localization is the consequence of irregular distribution of glycans forming the glycocalyx; 2) galectins are accumulated in the inner zone of the glycocalyx rather than at its outer face or directly in vicinity of the cell membrane; 3) patches are not associated with cell rafts.

Keywords

galectins glycans glycocalyx mammalian cell 

Abbreviations

biot

biotin

BSA

bovine serum albumin

CRD

carbohydrate recognition domain

CTB

cholera toxin B-sub-unit

DSA

agglutinin from Datura stramonium

FITC

fluorescein isothiocyanate

Gal

galectin

PBS

phosphate-buffered saline, pH 7.2

PBA

phosphate-buffered saline, pH 7.2, containing 0.2% BSA

RPE

R-phycoerythrin

Str

streptavidin

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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • E. M. Rapoport
    • 1
  • V. K. Matveeva
    • 1
  • O. A. Vokhmyanina
    • 1
  • I. M. Belyanchikov
    • 1
  • H.-J. Gabius
    • 2
  • N. V. Bovin
    • 1
  1. 1.Shemyakin and Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia
  2. 2.Faculty of Veterinary Medicine, Institute of Physiological ChemistryLudwig-Maximilians-UniversityMunichGermany

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