Abstract
The kinetics of protein synthesis was investigated in primary cultures of hepatocytes from old rats in serum–free medium. The rats were fed mixed fodder supplemented with glutamic acid and then transferred to a regular mixed fodder. The amplitude of protein synthesis rhythm in hepatocytes isolated from these rats increased on average 2–fold in comparison with the rats not receiving glutamic acid supplement. Based on this indicator reflecting the degree of cell–cell interactions, the cells from old rats were not different from those of young rats. The effect was preserved for 3–4 days. These results are discussed in connection with our previous data on preservation of the effect of single administration of gangliosides, noradrenaline, serotonin, and other synchronizers on various cell populations. In contrast to the other investigated factors, glutamic acid is capable of penetrating the blood–brain barrier, which makes its effect possible not only in the case of hepatocytes and other non–brain cells, but also in neurons.
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Brodsky, V. Y., Malchenko, L. A., Konchenko, D. S., Zvezdina, N. D., and Dubovaya, T. K. (2016) Glutamic acid–amino acid, neurotransmitter, and drug–is respon–sible for protein synthesis rhythm in hepatocyte populations in vitro and in vivo, Biochemistry (Moscow), 81, 892–898.
Brodsky, V. Y. (2014) Circahoralian (ultradian) metabolic rhythms, Biochemistry (Moscow), 79, 483–495.
Brodsky, V. Y., Malchenko, L. A., Butorina, N. N., Lazarev (Konchenko), D. S., Zvezdina, N. D., and Dubovaya, T. K. (2017) Glutamic acid as enhancer of protein synthesis kinetics in hepatocytes from old rats, Biochemistry (Moscow), 82, 957–961.
Ugryumov, M. V. (ed.) (2014) Neurodegenerative Diseases: from Genome to Entire Organism [in Russian], Vols. 1 and 2, Nauchnyi Mir, Moscow.
Brodsky, V. Y., Nechaeva, N. V., Zvezdina, N. D., Prokazova, N. V., Golovanova, N. K., Novikova, T. E., Gvasava, I. G., and Fateeva, V. I. (2000) Ganglioside–mediated synchronization of the protein synthesis activity in cultured hepatocytes, Cell Biol. Int., 24, 211–222.
Brodsky, V. Y., Nechaeva, N. V., and Prilutskii, V. I. (1973) Residual processes in the kinetics of cellular proteins’ content in parotid, Tsitologiya, 15, 177–182.
Mandelbrot, B. B. (1980) The Fractal Geometry of Nature, Freeman, NY.
Lloyd, A. L., and Lloyd, D. (1993) Hypothesis: the central oscillator of the circadian clock is a controlled chaotic attractor, BioSystem, 29, 77–85.
Bunde, A., and Havlin, S. (1994) Fractals in Science, Springer, New York.
Brodsky, V. Y., Boikov, P. Y., Nechaeva, N. V., Yurovitsky, Y. G., Novikova, T. E., Fateeva, V. I., and Shevchenko, N. A. (1992) The rhythm of protein synthesis does not depend on oscillations of ATP level, J. Cell Sci., 103, 363–370.
Brodsky, V. Y., Zvezdina, N. D., Nechaeva, N. V., Novikova, T. E., Gvasava, I. G., Fateeva, V. I., and Malchenko, L. A. (2005) Single shortterm signal that enhances cooperative activity of the old rat hepatocytes acts for several days, Cell Biol. Int., 29, 971–975.
Brodsky, V. Y., and Zvezdina, N. D. (2010) Melatonin as the most effective organizer of the protein synthesis rhythm in hepatocytes in vitro and in vivo, Cell Biol. Int., 34, 1199–1204.
Lloyd, D., and Kippert, F. (1987) A temperature–compensated ultradian clock explains temperature–dependent quantal cell cycle times, in Temperature and Animal Cells (Bowler, K., and Fuller, B. J., eds.) Cambridge University Press, pp. 135–155.
Lloyd, D., and Murray, D. B. (2005) Ultradian metronome: timekeeper for orchestration of cellular coherence, Trends Biochem. Sci., 30, 373–377.
Ferreira, G. M. N., Hammond, K. D., and Gilbert, D. A. (1994) Insulin stimulation of high frequency phosphorylation dynamics in murine erythroleukemia cells, Biosystems, 32, 183–190.
Hammond, K. D., Bhoola, R., Bodalina, U., and Gilbert, D. A. (1998) Dynamic cells: temporal organization and control of phosphorylation, Trends Comp. Biochem. Physiol., 4, 75–88.
Avdonin, P. V., and Tkachuk, V. A. (1994) Receptors and Intracellular Calcium [in Russian], Nauka, Moscow.
Voronezhskaya, E. E., Khabarova, M. Y., Nezlin, L. P., and Ivashkin, E. G. (2012) Delayed action of serotonin in molluscan development, Acta Biol. Hung., 63, 210–216.
Ivashkin, E., Khabarova, M. Y., Melnikova, V., Nezlin, L. P., Kharchenko, O., Voronezhskaya, E. E., and Adameyko, I. (2015) Serotonin mediates maternal effects and directs developmental and behavioral changes in the progeny of snails, Cell Rep., 12, 1–15.
Boradinova, A. A., Zuzina, A. B., and Balaban, P. M. (2017) Role of atypical protein kinases in maintenance of long–term memory and synaptic plasticity, Biochemistry (Moscow), 82, 243–256.
Brodsky, V. Y., Konchenko, D. S., Zvezdina, N. D., Malchenko, L. A., and Dubovaja, T. K. (2012) Unlike norepinephrine and serotonin, dopamine disorganizes direct cell–cell communica–tion in hepatocyte cultures, J. Cell Tissue Res., 12, 3265–3271.
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Original Russian Text © V. Y. Brodsky, L. A. Malchenko, D. S. Lazarev, N. N. Butorina, T. K. Dubovaya, N. D. Zvezdina, 2018, published in Biokhimiya, 2018, Vol. 83, No. 3, pp. 427–432.
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Brodsky, V.Y., Malchenko, L.A., Lazarev, D.S. et al. Glutamic Acid Signal Synchronizes Protein Synthesis Kinetics in Hepatocytes from Old Rats for the Following Several Days. Cell Metabolism Memory. Biochemistry Moscow 83, 294–298 (2018). https://doi.org/10.1134/S0006297918030094
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DOI: https://doi.org/10.1134/S0006297918030094