Abstract
MicroRNAs are involved in many biological processes. Studying microRNA function requires genetic strategies generating loss-of-function phenotypes, especially in vivo. However, few microRNA loss-of-function models have been reported in mice. Here, we generated several transgenic mouse lines to stably and specifically knockdown miR-483-5p by overexpressing microRNA sponges from CAG promoters. The different levels of expression of microRNA sponges resulted in different levels of mature miR-483-5p, which upregulated serum ALT/AST in these transgenic lines. These results indicate microRNA sponges are effective in mice in vivo, and they can be used in microRNA loss-of-function research.
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Abbreviations
- ALT:
-
alanine aminotransferase
- AST:
-
aspartate aminotransferase
- HE:
-
hematoxylin-eosin (staining)
- LOF:
-
loss-of-function (research)
- miRNAs:
-
microRNAs
- UTR:
-
untranslated region
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Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM17-325, November 6, 2017.
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Zhang, Y., Wu, D., Zhang, X. et al. MicroRNA sponge knockdowns miR-483-5p and upregulates serum ALT/AST in transgenic mice. Biochemistry Moscow 83, 54–59 (2018). https://doi.org/10.1134/S0006297918010078
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DOI: https://doi.org/10.1134/S0006297918010078