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Biochemistry (Moscow)

, Volume 82, Issue 11, pp 1324–1335 | Cite as

Factors beyond enolase 2 and mitochondrial lysyl-tRNA synthetase precursor are required for tRNA import into yeast mitochondria

  • M. V. Baleva
  • M. Meyer
  • N. Entelis
  • I. Tarassov
  • P. Kamenski
  • B. Masquida
Article

Abstract

In yeast, the import of tRNALys with CUU anticodon (tRK1) relies on a complex mechanism where interaction with enolase 2 (Eno2p) dictates a deep conformational change of the tRNA. This event is believed to mask the tRNA from the cytosolic translational machinery to re-direct it towards the mitochondria. Once near the mitochondrial outer membrane, the precursor of the mitochondrial lysyl-tRNA synthetase (preMsk1p) takes over enolase to carry the tRNA within the mitochondrial matrix, where it is supposed to participate in translation following correct refolding. Biochemical data presented in this report focus on the role of enolase. They show that despite the inability of Eno2p alone to form a complex with tRK1, mitochondrial import can be recapitulated in vitro using fractions of yeast extracts sharing either recombinant or endogenous yeast Eno2p as one of the main components. Taken together, our data suggest the existence of a protein complex containing Eno2p that is involved in RNA mitochondrial import.

Keywords

RNA mitochondrial import RNA chaperone tRNA–enolase interaction enolase moonlighting protein 

Abbreviations

EMSA

electrophoretic mobility shift assay

Eno2p

enolase 2

ITC

isothermal titration microcalorimetry

preMsk1p

precursor of mitochondrial lysyl-tRNA synthetase

tRK1

tRNALys with CUU anticodon

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Copyright information

© Pleiades Publishing, Ltd. 2017

Authors and Affiliations

  • M. V. Baleva
    • 1
    • 2
  • M. Meyer
    • 3
  • N. Entelis
    • 1
  • I. Tarassov
    • 1
  • P. Kamenski
    • 2
    • 4
  • B. Masquida
    • 1
  1. 1.GMGMCNRS − University of StrasbourgStrasbourgFrance
  2. 2.Lomonosov Moscow State UniversityFaculty of BiologyMoscowRussia
  3. 3.IGBMCCNRS − University of StrasbourgIllkirchFrance
  4. 4.Immanuil Kant Baltic Federal UniversityInstitute of Living SystemsKaliningradRussia

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