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Nigericin inhibits epithelial ovarian cancer metastasis by suppressing the cell cycle and epithelial−mesenchymal transition

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Abstract

Epithelial ovarian cancer (EOC) has the highest mortality among various types of gynecological malignancies. Most patients die of metastasis and recurrence due to cisplatin resistance. Thus, it is urgent to develop novel therapies to cure this disease. CCK-8 assay showed that nigericin exhibited strong cytotoxicity on A2780 and SKOV3 cell lines. Flow cytometry indicated that nigericin could induce cell cycle arrest at G0/G1 phase and promote cell apoptosis. Boyden chamber assay revealed that nigericin could inhibit migration and invasion in a dose-dependent manner by suppressing epithelial–mesenchymal transition (EMT) in EOC cells. These effects were mediated, at least partly, by the Wnt/β-catenin signaling pathway. Our results demonstrated that nigericin could inhibit EMT during cell invasion and metastasis through the canonical Wnt/β-catenin signaling pathway. Nigericin may prove to be a novel therapeutic strategy that is effective in patients with metastatic EOC.

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Abbreviations

EMT:

epithelial–mesenchymal transition

EOC:

epithelial ovarian cancer

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan, Shandong, 250012, P.R. China

    Wen Wang, Yan Zhao, Wenying Pan, Wenqian Huang, Taotao Dong & Shiqian Zhang

  2. Department of Obstetrics and Gynecology, Tengzhou Central People’s Hospital, Tengzhou, Shandong, 277500, P.R. China

    Wen Wang, Shujuan Yao & Xiujuan Cui

  3. Department of Obstetrics and Gynecology, Linyi People’s Hospital, Linyi, Shandong, 276000, P.R. China

    Yan Zhao

  4. Department of Obstetrics and Gynecology, Binzhou Medical School Affiliated Hospital, Binzhou, Shandong, P.R. China

    Wenying Pan

  5. School of Life Science, Shandong University, Jinan, Shandong, P.R. China

    Jiangang Gao

Authors
  1. Wen Wang
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  2. Yan Zhao
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  3. Shujuan Yao
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  4. Xiujuan Cui
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  7. Jiangang Gao
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  8. Taotao Dong
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  9. Shiqian Zhang
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Additional information

Published in Russian in Biokhimiya, 2017, Vol. 82, No. 8, pp. 1209-1219.

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Wang, W., Zhao, Y., Yao, S. et al. Nigericin inhibits epithelial ovarian cancer metastasis by suppressing the cell cycle and epithelial−mesenchymal transition. Biochemistry Moscow 82, 933–941 (2017). https://doi.org/10.1134/S0006297917080089

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  • DOI: https://doi.org/10.1134/S0006297917080089

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