Biochemistry (Moscow)

, Volume 82, Issue 7, pp 816–820 | Cite as

Coupling of translation initiation and termination does not depend on the mode of initiation

  • E. A. Sogorin
  • G. K. Selikhanov
  • S. Ch. AgalarovEmail author


Recently we described a novel phenomenon observed during eukaryotic translation in a cell-free system: the coupling of initiation and termination on different mRNA molecules. Here we show that the phenomenon does not depend on a special mode of initiation. The mRNAs with certain leader sequences known to require different determinants for successful initiation were examined. Even in a case of using the intergenic internal ribosome entry site (IRES) of cricket paralysis virus RNA as the leader sequence, while no initiation factors are required, the effect of coupling is well expressed, including trials in the presence of hippuristanol as an inhibitor of eIF4A. Thus, the effect persists in the absence of scanning and does not depend on initiator tRNA and eIF2. The results suggest that the initiation factors are not involved in the coupling mechanism.


translation initiation translation termination initiation factors hippuristanol 



internal ribosome entry site


untranslated region


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Jackson, R. J., Hellen, C., and Pestova, T. V. (2012) Termination and posttermination events in eukaryotic translation, Adv. Protein Chem. Struct. Biol., 86, 45–93.CrossRefPubMedGoogle Scholar
  2. 2.
    Dever, T. E., and Green, R. (2012) The elongation, termination, and recycling phases of translation in eukaryotes, Cold Spring Harb. Perspect. Biol., 4, 1–16.CrossRefGoogle Scholar
  3. 3.
    Kolb, V. A., Makeyev, E. V., and Spirin, A. S. (1994) Folding of firefly luciferase during translation in a cell-free system, EMBO J., 13, 3631–3637.PubMedPubMedCentralGoogle Scholar
  4. 4.
    Sogorin, E. A., Agalarov, S. C., and Spirin, A. S. (2016) Inter-polysomal coupling of termination and initiation during translation in eukaryotic cell-free system, Sci. Rep., 6, 24518.CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Sakharov, P. A., and Agalarov, S. C. (2016) Free initiation factors eIF4A and eIF4B are dispensable for translation initiation on uncapped mRNAs, Biochemistry (Moscow), 81, 1198–1204.CrossRefGoogle Scholar
  6. 6.
    Wilson, J. E., Pestova, T. V., Hellen, C. U., and Sarnow, P. (2000) Initiation of protein synthesis from the A site of the ribosome, Cell, 102, 511–520.CrossRefPubMedGoogle Scholar
  7. 7.
    Alekhina, O. M., Vassilenko, K. S., and Spirin, A. S. (2007) Translation of non-capped mRNAs in a eukaryotic cell-free system: acceleration of initiation rate in the course of polysome formation, Nucleic Acids Res., 35, 6547–6559.CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Kopeina, G. S., Afonina, Z. A., Gromova, K. V., Shirokov, V. A., Vasiliev, V. D., and Spirin, A. S. (2008) Step-wise formation of eukaryotic double-row polyribosomes and circular translation of polysomal mRNA, Nucleic Acids Res., 36, 2476–2488.CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Lyabin, D. N., Eliseeva, I. A., and Ovchinnikov, L. P. (2012) YB-1 synthesis is regulated by mTOR signaling pathway, PLoS One, 7, 1–11.CrossRefGoogle Scholar
  10. 10.
    Prokhorova, I. V., Akulich, K. A., Makeeva, D. S., Osterman, I. A., Yusupov, M. M., and Dmitriev, S. E. (2016) Amicoumacin A induces cancer cell death by targeting the eukaryotic ribosome, Sci. Rep., 6, 27720.CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Sogorin, E. A., Agalarov, S. C., and Spirin, A. S. (2015) Formation of new polysomes on free mRNAs in a cell-free translation systems is accompanied by partial disassembly of previously formed polysomes, Biochemistry (Moscow), 80, 1327–1330.CrossRefGoogle Scholar
  12. 12.
    Shirokov, V. A., Kommer, A., Kolb, V. A., and Spirin, A. S. (2007) Continuous-exchange protein-synthesizing sys-tems, Methods Mol. Biol., 375, 19–55.PubMedGoogle Scholar
  13. 13.
    Agalarov, S. C., Sakharov, P. A., Fattakhova, D. K., Sogorin, E. A., and Spirin, A. S. (2014) Internal translation initiation and eIF4F/ATP-independent scanning of mRNA by eukaryotic ribosomal particles, Sci. Rep., 4, 4438.CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Pestova, T. V., and Kolupaeva, V. G. (2002) The roles of individual eukaryotic translation initiation factors in ribosomal scanning and initiation codon selection, Genes Dev., 16, 2906–2922.CrossRefPubMedPubMedCentralGoogle Scholar
  15. 15.
    Bordeleau, M.-E., Mori, A., Oberer, M., Lindqvist, L., Chard, L. S., Higa, T., Belsham, G. J., Wagner, G., Tanaka, J., and Pelletier, J. (2006) Functional characterization of IRESes by an inhibitor of the RNA helicase eIF4A, Nat. Chem. Biol., 2, 213–220.CrossRefPubMedGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2017

Authors and Affiliations

  • E. A. Sogorin
    • 1
  • G. K. Selikhanov
    • 1
  • S. Ch. Agalarov
    • 1
    Email author
  1. 1.Institute of Protein ResearchRussian Academy of SciencesPushchino, Moscow RegionRussia

Personalised recommendations