Biochemistry (Moscow)

, Volume 82, Issue 5, pp 572–578 | Cite as

Minicircle kinetoplast genome of insect trypanosomatid Leptomonas pyrrhocoris

  • E. S. GerasimovEmail author
  • A. A. Gasparyan
  • I. A. Litus
  • M. D. Logacheva
  • A. A. KolesnikovEmail author


We present here the structure of a minicircle population based on transcriptome sequencing of Leptomonas pyrrhocoris. We show that minicircle DNA molecules are dimeric. As in dixenous species, the entire molecule of minicircle DNA is transcribed. This is the first minicircle transcriptome of monoxenous trypanosomatid species determined using NGS technology.


trypanosomatids kinetoplast minicircle DNA transcriptome assembly 



conservative region


conserved sequence block


guide RNA




kinetoplast DNA


kinetoplast RNA




next generation sequencing


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  1. 1.
    Yurchenko, V. Y., and Kolesnikov, A. A. (2001) Minicircular kinetoplast DNA of Trypanosomatidae, Mol. Biol. (Moscow), 35, 3–13.CrossRefGoogle Scholar
  2. 2.
    Lukes, J., Hashimi, H., and Zikova, A. (2005) Unexplained complexity of the mitochondrial genome and transcriptome in kinetoplastid flagellates, Curr. Genet., 48, 277–299.CrossRefPubMedGoogle Scholar
  3. 3.
    Simpson, L., Douglass, S. M., Lake, J. A., Pellegrini, M., and Li, F. (2015) Comparison of the mitochondrial genomes and steady state transcriptomes of two strains of the trypanosomatid parasite, Leishmania tarentolae, PLoS Negl. Trop. Dis., 7, e0003841.CrossRefGoogle Scholar
  4. 4.
    Koslovsky, D., Sun, Y., Hindenach, J., Theisen, T., and Lucas, J. (2014) The insectphase gRNA transcriptome in Trypanosoma brucei, Nucleic Acids Res., 42, 1873–1886.CrossRefGoogle Scholar
  5. 5.
    Maslov, D. A., Entelis, N. S., Kolesnikov, A. A., and Zaitseva, G. N. (1982) Kinetoplast DNA from Crithidia oncopelti. Restriction mapping of maxicircle DNA, Bioorg. Khim., 8, 676–685.Google Scholar
  6. 6.
    Pelletier, M., Read, L. K., and Afasizev, R. (2007) Isolation of RNA binding proteins involved in insertion/deletion editing, Methods Enzymol., 424, 75–105.CrossRefPubMedGoogle Scholar
  7. 7.
    Flegontov, P., Butenko, A., Firsov, S., Kraeva, N., Elias, M., Field, M. C., Filatov, D., Flegontova, O., Gerasimov, E. S., Hlavacova, J., Ishemgulova, A., Jackson, A. P., Kelly, S., Kostygov, A. Y., Logacheva, M. D., Maslov, D. A., Operdoes, F. R., O’Reilly, A., Sadlova, J., Sevcikova, T., Venkatesh, D., Vlcek, C., Volf, P., Votypka, J., Zahonova, K., Yurchenko, V., and Lukes, J. (2016) Genome of Leptomonas pyrrhocoris: a highquality reference for monoxenous trypanosomatids and new insights into evolution of Leishmania, Sci. Rep., 6.Google Scholar
  8. 8.
    Sugisaki, H., and Ray, D. S. (1987) DNA sequence of Crithidia fasciculate kinetoplast minicircles, Mol. Biochem. Parasitol., 23, 253–263.CrossRefPubMedGoogle Scholar
  9. 9.
    Yurchenko, V. Y., Kolesnikov, A. A., and Lukes, J. (2000) Phylogenetic analysis of Trypanosomatina (Protozoa: Kinetoplastida) based on minicircle conserved regions, Folia Parasitol., 47, 1–5.CrossRefPubMedGoogle Scholar
  10. 10.
    Baron, L. I., Yurchenko, V. Y., and Kolesnikov, A. A. (1995) Structure of minicircular kinetoplast DNA from trypanosome, a parasite of the river shark, Mol. Biol. (Moscow), 29, 1341–1348.Google Scholar
  11. 11.
    Fu, G., Lambson, B., and Barker, D. (1999) Characterization of kinetoplast DNA minicircles from Herpetomonas samuelpessoai, FEMS Microbiol. Lett., 172, 65–71.CrossRefPubMedGoogle Scholar
  12. 12.
    Singh, N., and Rastogi, A. K. (1999) Kinetoplast DNA minicircles of Leishmania donovani express a protein product, Biochim. Biophis. Acta, 1444, 263–268.CrossRefGoogle Scholar
  13. 13.
    Savill, N. J., and Higgs, P. G. (2000) Redundant and nonfunctional guide RNA genes in Trypanosoma brucei are a consequence of multiple genes per minicircle, Gene, 256, 245–252.CrossRefPubMedGoogle Scholar
  14. 14.
    Kostygov, A. Yu., Grybchuk-Ieremenko, A., Malysheva, M. N., Frolov, A. O., and Yurchenko, V. (2014) Molecular revision of the genus Wallaceina, Protist, 165, 594–604.CrossRefPubMedGoogle Scholar
  15. 15.
    Gerasimov, E. S., Efimova, N. S., and Kolesnikov, A. A. (2012) Three patterns of trypanosomatid cryptogene structural organization, Mol. Biol. (Moscow), 46, 612–621.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2017

Authors and Affiliations

  1. 1.Faculty of BiologyLomonosov Moscow State UniversityMoscowRussia
  2. 2.Belozersky Institute of Physico-Chemical BiologyLomonosov Moscow State UniversityMoscowRussia

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