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Attenuation of focal adhesion kinase reduces lipopolysaccharide-induced inflammation injury through inactivation of the Wnt and NF-κB pathways in A549 cells

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Abstract

Overall analysis and understanding of mechanisms are of great importance for treatment of infantile pneumonia due to its high morbidity and mortality worldwide. In this study, we preliminarily explored the function and mechanism of focal adhesion kinase (FAK) in regulation of inflammatory response induced by lipopolysaccharides in A549 cells. Flow cytometry, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, quantitative reverse transcription polymerase chain reaction, and Western blot analysis were used to explore the correlation of FAK expression with cell apoptosis, viability, and the inflammatory cytokine activity in A549 cells. The results showed that knockdown of FAK enhanced cell viability, suppressed apoptosis, and decreased inflammatory cytokine activity. In addition, downregulation of FAK could activate the Wnt and nuclear factor κB signaling pathways. These findings suggest that FAK might be involved in progression of infantile pneumonia and could be a new therapeutic target for this disease.

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Abbreviations

FAK:

focal adhesion kinase

IP:

infantile pneumonia

LPS:

lipopolysaccharide

MTT:

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (assay)

NF-κB:

nuclear factor κB

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Authors and Affiliations

  1. Jining No. 1 People’s Hospital, Department of Pediatrics, 272011, Jining, China

    D. Bai, S. Cong & L. P. Zhu

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  1. D. Bai
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  2. S. Cong
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  3. L. P. Zhu
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Corresponding author

Correspondence to L. P. Zhu.

Additional information

Published in Russian in Biokhimiya, 2017, Vol. 82, No. 4, pp. 611-619.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM16-295, December 12, 2016.

These authors contributed equally to this work.

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Bai, D., Cong, S. & Zhu, L.P. Attenuation of focal adhesion kinase reduces lipopolysaccharide-induced inflammation injury through inactivation of the Wnt and NF-κB pathways in A549 cells. Biochemistry Moscow 82, 446–453 (2017). https://doi.org/10.1134/S0006297917040058

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  • DOI: https://doi.org/10.1134/S0006297917040058

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