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Microbiota induces expression of tumor necrosis factor in postnatal mouse skin

  • Molecular and Cellular Mechanisms of Inflammation (Special Issue) Guest Editors S. A. Nedospasov and D. V. Kuprash
  • Published:
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Abstract

Tumor necrosis factor (TNF) is a pleiotropic cytokine that regulates many important processes in the body. TNF production in a physiological state supports the structure of lymphoid organs and determines the development of lymphoid cells in hematopoiesis. However, chronic TNF overexpression leads to the development of various autoimmune disorders. Sites of TNF production in the naive state remain unclear due to the lack of in vivo models. In the present study, we used TNF-2A-Kat reporter mice to monitor the expression of TNF in different tissues. Comparative analysis of tissue fluorescence in TNF-2A-Kat reporter mice and wild type mice revealed constitutive expression of TNF in the skin of naive adult mice. In the skin of TNF-2A-Kat reporter mouse embryos, no statistically significant differences in the expression of TNF compared to wild type animals were observed. Furthermore, we established that local depletion of microflora with topical antibiotics leads to a reduction in the fluorescence signal. Thus, we assume that the skin microflora is responsible for the expression of TNF in the skin of mice.

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Abbreviations

AMP:

antimicrobial peptides

IFN:

interferon

IL:

interleukin

TNF:

tumor necrosis factor

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Authors and Affiliations

  1. Nizhny Novgorod State Medical Academy, 603005, Nizhny Novgorod, Russia

    D. V. Yuzhakova, M. V. Shirmanova & E. V. Zagaynova

  2. Lobachevsky State University of Nizhny Novgorod, 603950, Nizhny Novgorod, Russia

    D. V. Yuzhakova, I. V. Astrakhantseva, E. A. Vasilenko, E. N. Gorshkova, S. A. Nedospasov & A. A. Kruglov

  3. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991, Moscow, Russia

    A. A. Bocharov, M. S. Drutskaya & S. A. Nedospasov

  4. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991, Moscow, Russia

    S. A. Nedospasov & A. A. Kruglov

  5. Faculty of Biology, Lomonosov Moscow State University, 119991, Moscow, Russia

    S. A. Nedospasov & A. A. Kruglov

Authors
  1. D. V. Yuzhakova
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  2. M. V. Shirmanova
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  3. A. A. Bocharov
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  6. E. N. Gorshkova
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  7. M. S. Drutskaya
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  8. E. V. Zagaynova
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  9. S. A. Nedospasov
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  10. A. A. Kruglov
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Correspondence to A. A. Kruglov.

Additional information

Original Russian Text © D. V. Yuzhakova, M. V. Shirmanova, A. A. Bocharov, I. V. Astrakhantseva, E. A. Vasilenko, E. N. Gorshkova, M. S. Drutskaya, E. V. Zagaynova, S. A. Nedospasov, A. A. Kruglov, 2016, published in Biokhimiya, 2016, Vol. 81, No. 11, pp. 1553–1558.

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Yuzhakova, D.V., Shirmanova, M.V., Bocharov, A.A. et al. Microbiota induces expression of tumor necrosis factor in postnatal mouse skin. Biochemistry Moscow 81, 1303–1308 (2016). https://doi.org/10.1134/S0006297916110080

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  • DOI: https://doi.org/10.1134/S0006297916110080

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