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Participation of two carbonic anhydrases of the alpha family in photosynthetic reactions in Arabidopsis thaliana

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Abstract

The expression of genes of two carbonic anhydrases (CA) belonging to the a-family, α-CA2 and α-CA4 (according to the nomenclature in N. Fabre et al. (2007) Plant Cell Environ., 30, 617-629), was studied in arabidopsis (Arabidopsis thaliana, var. Columbia) leaves. The expression of the At2g28210 gene coding α-CA2 decreased under increase in plant illumination, while the expression of the At4g20990 gene coding α-CA4 increased. Under conditions close to optimal for photosynthesis, in plants with gene At2g28210 knockout, the effective quantum yield of photosystem 2 and the light-induced accumulation of hydrogen peroxide in leaves were lower than in wild type plants, while the coefficient of non-photochemical quenching of leaf chlorophyll a fluorescence and the rate of CO2 assimilation in leaves were higher. In plants with At4g20990 gene knockout, the same characteristics changed in opposite ways relative to wild type. Possible mechanisms of the participation of αa-CA2 and α-CA4 in photosynthetic reactions are discussed, taking into account that protons can be either consumed or released in the reactions they catalyze.

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Abbreviations

CA:

carbonic anhydrase

Chl:

chlorophyll

PAR:

photosynthetically active radiation

PETC:

photosynthetic electron-transport chain

PS1:

photosystem 1

PS2:

photosystem 2

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Authors and Affiliations

  1. Institute of Basic Biological Problems, Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Russia

    E. M. Zhurikova, L. K. Ignatova, N. N. Rudenko, V. A. Mudrik, D. V. Vetoshkina & B. N. Ivanov

Authors
  1. E. M. Zhurikova
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  2. L. K. Ignatova
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  3. N. N. Rudenko
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  4. V. A. Mudrik
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  5. D. V. Vetoshkina
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  6. B. N. Ivanov
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Corresponding author

Correspondence to B. N. Ivanov.

Additional information

Original Russian Text © E. M. Zhurikova, L. K. Ignatova, N. N. Rudenko, V. A. Mudrik, D. V. Vetoshkina, B. N. Ivanov, 2016, published in Biokhimiya, 2016, Vol. 81, No. 10, pp. 1463–1470.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM16-224, September 5, 2016.

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Zhurikova, E.M., Ignatova, L.K., Rudenko, N.N. et al. Participation of two carbonic anhydrases of the alpha family in photosynthetic reactions in Arabidopsis thaliana . Biochemistry Moscow 81, 1182–1187 (2016). https://doi.org/10.1134/S0006297916100151

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  • DOI: https://doi.org/10.1134/S0006297916100151

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