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Overview of fusion tags for recombinant proteins

Abstract

Virtually all recombinant proteins are now prepared using fusion domains also known as “tags”. The use of tags helps to solve some serious problems: to simplify procedures of protein isolation, to increase expression and solubility of the desired protein, to simplify protein refolding and increase its efficiency, and to prevent proteolysis. In this review, advantages and disadvantages of such fusion tags are analyzed and data on both well-known and new tags are generalized. The authors own data are also presented.

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Abbreviations

GST:

glutathione S-transferase

hG-CSF:

human granulocyte colony-stimulating factor

hGM-CSF:

human granulocyte-macrophage colony-stimulating factor

hIFN:

human interferon

MBP:

maltose-binding protein

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Correspondence to E. N. Kosobokova.

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Original Russian Text © E. N. Kosobokova, K. A. Skrypnik, V. S. Kosorukov, 2016, published in Biokhimiya, 2016, Vol. 81, No. 3, pp. 299–314.

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Kosobokova, E.N., Skrypnik, K.A. & Kosorukov, V.S. Overview of fusion tags for recombinant proteins. Biochemistry Moscow 81, 187–200 (2016). https://doi.org/10.1134/S0006297916030019

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  • DOI: https://doi.org/10.1134/S0006297916030019

Key words

  • fusion tags
  • chromatography
  • enhancement of protein solubility