Biochemistry (Moscow)

, Volume 80, Issue 10, pp 1327–1330 | Cite as

Formation of new polysomes on free mRNAs in a cell-free translation systems is accompanied by partial disassembly of previously formed polysomes

  • E. A. Sogorin
  • S. Ch. Agalarov
  • A. S. Spirin
Short Communication


A method for detection of the fluorescence-labeled mRNA in translating ribosomal complexes has been developed. It is demonstrated that in the working cell-free translation system with preformed polysomes, formation of new polysomes on free mRNA takes place. For the first time, it is shown that the process is accompanied by partial disassembly of the previously formed polysomes. This result is interpreted as an indication of the direct relationship between processes of translation termination of polysomal ribosomes and translation initiation of free mRNAs.


eukaryotic polyribosomes mRNA cell-free translation system fluorescence 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Shirokov, V. A., Kommer, A., Kolb, V. A., and Spirin, A. S. (2007) in Methods in Molecular Biology, Vol. 375 (Grandi, G., ed.) Humana Press Inc., Totowa, NJ, pp. 19–55.PubMedGoogle Scholar
  2. 2.
    Afonina, Z. A., Myasnikov, A. G., Shirokov, V. A., Klaholz, B. P., and Spirin, A. S. (2015) Conformation transitions of eukaryotic polyribosomes during multi-round translation, Nucleic Acids Res., 43, 618–628.PubMedCentralCrossRefPubMedGoogle Scholar
  3. 3.
    Spirin, A. S., Baranov, V. I., Ryabova, L. A., Ovodov, S. Y., and Alakhov, Y. B. (1988) A continuous cell-free translation system capable of producing polypeptides in high yield, Scienc., 242, 1162–1164.CrossRefGoogle Scholar
  4. 4.
    Madin, K., Sawasaki, T., Ogasawara, T., and Endo, Y. (2000) A highly efficient and robust cell-free protein syn-thesis system prepared from wheat embryos: plants appar-ently contain a suicide system directed at ribosomes, Proc. Natl. Acad. Sci. US., 97, 559–564.CrossRefGoogle Scholar
  5. 5.
    Alekhina, O. M., Vassilenko, K. S., and Spirin, A. S. (2007) Translation of non-capped mRNAs in a eukaryotic cell free system: acceleration of initiation rate in the course of polysome formation, Nucleic Acids Res., 35, 6547–6559.PubMedCentralCrossRefPubMedGoogle Scholar
  6. 6.
    Pokrovskaya, I. D., and Gurevich, V. V. (1994) In vitro tran-scription: preparative RNA yields in analytical scale reac-tions, Anal. Biochem., 220, 420–423.CrossRefPubMedGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • E. A. Sogorin
    • 1
  • S. Ch. Agalarov
    • 1
  • A. S. Spirin
    • 1
  1. 1.Institute of Protein ResearchRussian Academy of SciencesPushchino, Moscow RegionRussia

Personalised recommendations