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Epitope mapping of lymphocyte phosphatase-associated phosphoprotein

Biochemistry (Moscow) volume 79, pages 1397–1404 (2014)Cite this article

Abstract

Lymphocyte phosphatase-associated phosphoprotein (LPAP) is a transmembrane protein with unknown function. The available data on its close association with phosphatase CD45 and its phosphorylation depending on cell activation suggest that LPAP can play a significant role in the antigenic stimulation of lymphocytes. We have localized three antigenic epitopes of the LPAP molecule that can be detected using monoclonal antibodies prepared earlier. Experiments on reactions of antibodies with point mutants and shortened forms of the LPAP protein revealed regions of the amino acid sequence that correspond to the epitopes recognized by the antibodies.

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Abbreviations

BSA:

bovine serum albumin

CD45-AP:

CD45-associated protein

FITC:

fluorescein isothiocyanate

KLH:

keyhole limpet hemocyanin

LPAP:

lymphocyte phosphatase-associated phosphoprotein

PBS:

phosphate buffered saline

PMSF:

phenylmethylsulfonyl fluoride

R6G-SE:

rhodamine 6G succinimidyl ester

SDS:

sodium dodecyl sulfate

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Affiliations

  1. Institute of Immunology, Federal Medical-Biological Agency, Kashirskoe Shosse 24/2, 115478, Moscow, Russia

    A. V. Filatov & D. V. Mazurov

  2. Faculty of Biology, Lomonosov Moscow State University, 119234, Moscow, Russia

    T. D. Meshkova

Authors
  1. A. V. Filatov
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  2. T. D. Meshkova
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  3. D. V. Mazurov
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Corresponding author

Correspondence to A. V. Filatov.

Additional information

Original Russian Text © A. V. Filatov, T. D. Meshkova, D. V. Mazurov, 2014, published in Biokhimiya, 2014, Vol. 79, No. 12, pp. 1705–1714.

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Filatov, A.V., Meshkova, T.D. & Mazurov, D.V. Epitope mapping of lymphocyte phosphatase-associated phosphoprotein. Biochemistry Moscow 79, 1397–1404 (2014). https://doi.org/10.1134/S0006297914120153

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  • DOI: https://doi.org/10.1134/S0006297914120153

Kew words

  • LPAP
  • monoclonal antibodies
  • site directed mutagenesis
  • human lymphocytes