Abstract
Results of expression analysis of transcription of the flamenco locus that controls transposition of the mobile genetic element gypsy, RNA interference system genes ago3, zuc, aub, and HP1 heterochromatin protein family genes hp1a, hp1b, hp1c, hp1d (rhino), and hp1e in D. melanogaster SS strain mutant on the flamenco gene are presented. We show that the number of transcripts in the SS strain that are formed in the flamenco locus is unchanged in some freely chosen points, and this is different from the wild-type strain where a decreased number of transcripts is observed, which clearly is a result of processing of the flamenco locus primary transcript, a predecessor of piRNA. At the same time, expression of genes of the RNA interference system is not affected, but there is a reduced level of hp1d gene expression in ovary tissue. We suggest that the hp1d gene product is directly or indirectly involved in the flamenco locus primary transcript processing.
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Abbreviations
- HP1:
-
heterochromatin protein 1
- MGE:
-
mobile genetic element
- piRNA:
-
small mRNA associated with protein PIWI
References
Lippman, Z., and Martienssen, R. (2004) The role of RNA interference in heterochromatic silencing, Nature, 431, 364–370.
Moshkovich, N., and Lei, E. P. (2010) HP1 recruitment in the absence of argonaute proteins in Drosophila, PLoS Genet., 6, e1000880.
Kwon, S. H., and Workman, J. L. (2011) HP1c casts light on dark matter, Cell Cycle, 10, 625–630.
Lee, D. H., Li, Y., Shin, D.-H., Sang, A. Y., Bang, S.-Y., Eun Kyung Park, Han, J.-W., and Kwon, S. H. (2013) DNA microarray profiling of genes differentially regulated by three heterochromatin protein 1 (HP1) homologs in Drosophila, Biochem. Biophys. Res. Commun., 434, 820–828.
Klattenhoff, C., Xi, H., Li, C., Lee, S., Xu, J., Khurana, J. S., Zhang, F., Schultz, N., Koppetsch, B. S., Nowosielska, A., Seitz, H., Zamore, P. D., Weng, Z., and Theurkauf, W. E. (2009) The Drosophila HP1 homolog rhino is required for transposon silencing and piRNA production by dualstrand clusters, Cell, 138, 1137–1149.
Vermaak, D., and Malik, H. S. (2009) Multiple roles for heterochromatin protein 1 genes in Drosophila, Ann. Rev. Genet., 43, 467–492.
Castel, S. E., and Martienssen, R. A. (2013) RNA interference in the nucleus: roles for small RNAs in transcription, epigenetics and beyond, Nat. Rev. Genet., 14, 100–112.
Minervini, C. F., Marsano, R. M., Casieri, P., Fanti, L., Caizzi, R., Pimpinelli, S., Rocchi, M., and Viggiano, L. (2007) Heterochromatin protein 1 interacts with 5′UTR of transposable element ZAM in a sequence-specific fashion, Gene, 393, 1–10.
Brower-Toland, B., Findley, S. D., Jiang, L., Liu, L., Yin, H., Dus, M., Zhou, P., Elgin, S. C. R., and Lin, H. (2007) Drosophila PIWI associates with chromatin and interacts directly with HP1a, Genes Dev., 21, 2300–2311.
Nefedova, L. N., Romanova, N. I., and Kim, A. I. (2007) Peculiarities of DIP1 gene structural organization in strains of Drosophila melanogaster, mutant at the flamenco gene, Genetika, 43, 71–78.
Brennecke, J., Aravin, A. A., Stark, A., Dus, M., Kellis, M., Sachidanandam, R., and Hannon, G. J. (2007) Discrete small RNA-generating loci as master regulators of transposon activity in Drosophila, Cell, 128, 1089–1103.
Mevel-Ninio, M., Pelisson, A., Kinder, J., Campos, A. R., and Bucheton, A. (2007) The flamenco locus controls the gypsy and ZAM retroviruses and is required for Drosophila oogenesis, Genetics, 175, 1615–1624.
Desset, S., Meignin, C., Dastugue, B., and Vaury, C. (2003) COM, a heterochromatic locus governing the control of independent endogenous retroviruses from Drosophila melanogaster, Genetics, 164, 501–509.
Prud’homme, N., Kim, A., Bucheton, A., and Pelisson, A. (2001) Characterization of the flamenco region of the Drosophila melanogaster genome, Genetics, 158, 701–713.
Nefedova, L. N., Urusov, F. A., Romanova, N. I., Shmelkova, A. O., and Kim, A. I. (2012) Investigation of transcriptional and transposon activity of the Tirant retrotransposon in Drosophila melanogaster strains, mutant at the flamenco locus, Genetika, 48, 1089–1096.
Goriaux, C., Desset, S., Renaud, Y., Vaury, C., and Brasset, E. (2014) Transcriptional properties and splicing of the flamenco piRNA cluster, EMBO Rep., 15, 411–418.
Urusov, F. A., Nefedova, L. N., Lavrenov, A. R., Romanova, N. I., and Kim, A. I. (2013) Genetic and molecular analysis of loci complementation, Vavilov Zh. Genet. Selek., 17, 381–389.
Kellum, R., and Alberts, B. M. (1995) Heterochromatin protein 1 is required for correct chromosome segregation in Drosophila embryos, J. Cell Sci., 108, 1419–1431.
Nishimasu, H., Ishizu, H., Saito, K., Fukuhara, S., Kamatani, M. K., Bonnefond, L., Matsumoto, N., Nishizawa, T., Nakanaga, K., Aoki, J., Ishitani, R., Siomi, H., Siomi, M. C., and Nureki, O. (2012) Structure and function of Zucchini endoribonuclease in piRNA biogenesis, Nature, 491, 284–287.
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Original Russian Text © A. R. Lavrenov, L. N. Nefedova, N. I. Romanova, A. I. Kim, 2014, published in Biokhimiya, 2014, Vol. 79, No. 11, pp. 1554–1560.
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Lavrenov, A.R., Nefedova, L.N., Romanova, N.I. et al. Expression of hp1 family genes and their plausible role in formation of flamenco phenotype in D. melanogaster . Biochemistry Moscow 79, 1267–1272 (2014). https://doi.org/10.1134/S0006297914110157
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DOI: https://doi.org/10.1134/S0006297914110157