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Iron metabolism after application of modified magnetite nanoparticles in rats

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Abstract

The influence of modified nanosized magnetite (NSM) particles (magnetic microspheres coated with chitosan and magnetoliposomes) after a single intravenous infusion of their suspensions on iron metabolism in rats has been studied. Modern physical and chemical methods (X-ray fluorescence, dynamic light scattering, transmission electron microscopy) were used for standardization of the modified NSM particles (their size, structure, ζ-potential, and concentration were determined). Atomic emission spectroscopy was used to reveal the dynamics of iron content in rat liver, spleen, lungs, and kidneys during 120 days. Colorimetric and immunoturbidimetric methods were used to determine the concentrations of plasma iron and the proteins involved in its metabolism — ceruloplasmin, transferrin, and ferritin. Their dynamics through- out the experiments were studied.

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Abbreviations

NSM:

nanosized magnetite particles

MNP:

mononuclear phagocytes

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Authors and Affiliations

  1. Siberian State Medical University, Moskovsky Tract 2, 634050, Tomsk, Russia

    I. V. Milto, T. K. Klimenteva, I. V. Suhodolo, G. Yu. Vasukov & V. V. Ivanova

  2. National Research Tomsk Polytechnic University, pr. Lenina 36, 634050, Tomsk, Russia

    I. V. Milto

  3. Research Institute of Molecular Biology and Biophysics, Siberian Branch of the Russian Academy of Medical Sciences, ul. Timakova 2/12, 630117, Novosibirsk, Russia

    A. Yu. Grishanova

Authors
  1. I. V. Milto
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  2. A. Yu. Grishanova
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  3. T. K. Klimenteva
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  4. I. V. Suhodolo
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  5. G. Yu. Vasukov
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  6. V. V. Ivanova
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Correspondence to I. V. Milto.

Additional information

Original Russian Text © I. V. Milto, A. Yu. Grishanova, T. K. Klimenteva, I. V. Suhodolo, G. Yu. Vasukov, V. V. Ivanova, 2014, published in Biokhimiya, 2014, Vol. 79, No. 11, pp. 1527–1538.

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Milto, I.V., Grishanova, A.Y., Klimenteva, T.K. et al. Iron metabolism after application of modified magnetite nanoparticles in rats. Biochemistry Moscow 79, 1245–1254 (2014). https://doi.org/10.1134/S0006297914110121

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  • DOI: https://doi.org/10.1134/S0006297914110121

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